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RAPD study on some common species ofPorphyra in China |
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| Authors: | Mei Kuang Su-juan Wang Yao Li Da-leng Shen Cheng-kui Zeng |
| Institution: | 1.Shanghai Fisheries University, 200090, Shanghai, China ;2.Fudan University, 200433, Shanghai, China ;3.Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China ; |
| Abstract: | Electroporation, PEC, PEG plus electroporation and Biolistics methods were tested in gene transformation ofP. yezoensis. The exogenousgus was from plasmid of pBI121 and pCAMBIA1301, both contain the CaMV35S promoter. The receptors included the protoplasts, tissues and free-living conchocelis filaments ofP. yezoensis. Several factors, for example, the voltage, capacitance and bivalent cations, etc., were studied. Results show that these four methods are all efficient for gene transformation inP. yezoensis; and that PEG is the best one, with transformation efficiency of up to 4×10?5. GUS activity was detected 26 days after transformation by using PEG method. |
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