| Development and Characterization of Microsatellite Markers for the Pacific Abalone （Haliotis discus） via EST Database Mining |
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| 作者单位： | [1]Laboratory of Marine Genetics and Breeding （MGB）, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China [2]Shangdong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, P R. China |
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| 基金项目： | The work is mainly 国家自然科学基金，Grant of the Inspection Technologies for Human and Animal Resources，the Student Research Training Program |
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| 摘 要： |
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| 关 键 词： | 鲍鱼 软体动物 太平洋 微卫星 |
| 收稿时间： | 14 July 2010 |
Development and characterization of microsatellite markers for the Pacific abalone (<Emphasis Type="Italic">Haliotis discus</Emphasis>) via EST database mining |
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| Authors: | Aibin Zhan Zhenmin Bao Mingling Wang Dan Chang Jian Yuan Xiaolong Wang Xiaoli Hu Chengzhu Liang Jingjie Hu |
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| Institution: | 1. Laboratory of Marine Genetics and Breeding (MGB), College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China
2. Shangdong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, P. R. China |
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| Abstract: | The EST database of the Pacific abalone (Haliotis discus) was mined for developing mierosatellite markers. A total of 1476 EST sequences were registered in GenBank when data mining was performed. Fifty sequences (approximately 3.4%) were found to contain one or more mierosatellites. Based on the length and GC content of the flanking regions, duster analysis and BLASTN, 13 microsatellite-containing ESTs were selected for PCR primer design. The results showed that 10 out of 13 primer pairs could amplify seorable PCR products and showed polymorphism. The number of alleles ranged from 2 to 13 and the values of Hoand He varied from 0.1222 to 0.8611 and 0.2449 to 0.9311, respectively. No significant linkage disequilibrium (LD) between any pairs of these loci was found, and 6 of 10 loci conformed to the Hardy-Weinberg equilibrium (HWE). These EST-SSRs are therefore potential tools for studies of intraspecies variation and hybrid identification. |
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| Keywords: | mierosatellite Pacific abalone Haliotis discus EST |
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