Response of microbial community to petroleum stress and phosphate dosage in sediments of Jiaozhou Bay,China |
| |
| Authors: | Yangguo Zhao Min Chen Jie Bai Xinwei Li Farhana Zulfiqar Qianli Wang |
| |
| Affiliation: | 1. College of Environmental Science and Engineering, Ocean University of China, Qingdao, 266100, P. R. China
2. Key Laboratory of Marine Environment and Ecology, Ministry of Education, Qingdao, 266100, P. R. China
|
| |
| Abstract: | The dynamic microcosms were used to evaluate the effect of oil spills on microbial ecological system in marine sediment and the enhancement of nutrient on the oil removal. The function and structure of microbial community caused by the oil pollution and phosphate dosage were simultaneously monitored by dehydrogenase activity assay and PCR-denaturing gradient gel electrophoresis (DGGE) techniques. The results indicated that the amount of total bacteria in all dynamic microcosms declined rapidly with incubation time. The number of petroleum-degrading bacteria and the activity of sediment dehydrogenase were gradually enhanced by petroleum in the oil-treated microcosms, while they both showed no obvious response to phosphate dosage. In comparison, phosphate spiked heterotrophic bacteria and they showed a significant increase in amount. DGGE profiles indicated that petroleum dosage greatly changed community structure, and the bacteria belonged to class Deltaproteobacteria, and phyla Bacteroidetes and Chlorobi were enriched. This study demonstrated that petroleum input greatly impacted the microbial community structure and consequently the marine sediment petroleum-degrading activity was enhanced. Phosphate dosage would multiply heterotrophic bacteria but not significantly enhance the petroleum degradation. |
| |
| Keywords: | marine sediments oil spills dehydrogenase activity phosphate heterotrophic bacteria bacterial community |
| 本文献已被 CNKI SpringerLink 等数据库收录！ |
|
