极地寡营养细菌AT52(Alteromonas stellipolarissp.nov.)超微结构、生长生理特性及液态富营养发酵培养条件优化

寡营养细菌是一类能利用低浓度有机营养基质的细菌,通过研究1株分离自南极海洋的极地寡营养细菌AT52,发现富营养条件下AT52菌体细胞周身附肢,附肢长短不一,具鞭毛,菌体出芽生殖,由一层原生质膜包围.寡营养饥饿生存的菌体细胞相对较小,近球形,边缘不平滑,无鞭毛附肢等超微结构,细胞体眠未见出芽生殖过程.实验中采用寡营养环境...

THE ULTRASTRUCTURE, PHYSIOLOGICAL CHARACTERISTICS AND OPTIMIZED NUTRITIOUS LIQUID FERMENTATION CONDITION OF OLIGOTROPHIC BACTERIA AT52(ALTEROMONAS STELLIPOLARIS SP.NOV.)

Oligotrophic bacteria are a class can take advantage of low concentrations of organic nutrients matrix of bacteria, Oligotrophic bacteria AT52 is a strain isolated from the Antarctic polar ocean,its found AT52 cell have appendages, with flagella, cell budding , appendages of different lengths, surrounded by a layer of plasma membrane under the conditions in deep nutrient-rich. Oligotrophic bacterial cells to survive starvation is relatively small, nearly spherical, the edge is not smooth, without appendages such as flagella ultrastructure of cells as non-dormant buds of reproductive processes.This experiment studied the growth physiological characteristic of AT52,separately,in oligotrophic environment and rich nutrition environment.The optimal matrix: Poptone 5g,Yeast Extract 1g,the Antarctic seawater 1000mL.The optimal conditions: temperature 12℃,pH 8.0,rotation speed 180r/min, inoculation density 5％ and 200mL liquid medium joined into the 500mL Triangle bottle to ensure enough oxygen. AT52’s production cycle was shorted by thses conditions which provides the basis for industrial production.