| Characterization of SNAP-25 gene from marine teleostean, Lateolabrax japonicus |
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| 作者姓名： | 陈魁 黄晓航 柴迎梅 |
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| 作者单位： | Institute of Oceanology Chinese Academy of Sciences，The First Institute of Oceanography State Oceanic Administration，The First Institute of Oceanography State Oceanic Administration，Department of Physiology University of Toronto Toronto M5S 1A8 Canada，Qingdao 266071 China The First Institute of Oceanography State Oceanic Administration Qingdao 266061 China Graduate University Chinese Academy of Sciences Beijing 100039 China，Qingdao 266061 China，Qingdao 266061 China |
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| 基金项目： | 国家自然科学基金;国家高技术研究发展计划(863计划) |
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| 摘 要： | The t-SNARE protein SNAP-25 (synaptosome-associated protein of 25 kDa) plays an essential role in regulating fusion between the vesicle and plasma membranes during exocytosis. To clone and characterize SNAP-25 gene, the first step in the functional study of SNARE proteins in marine teleostean, was to obtain the cDNA of sea perch SNAP-25 (SPsn25) by RT-PCR and RACE-PCR amplification of a Japanese sea perch. The full-length cDNA of 831bp contains a CDS of 615 bp, coding 204 amino acid residues, and a 5′UTR of 219bp. Bioinformatic analysis revealed that SPsn25 corresponds with SNAP-25a isoform and shares 91.1% identity with SNAP-25a of a goldfish and a zebrafish. The SPsn25 expression in both mRNA and protein levels in the Japanese sea perch had been identified through semi-quantitative RT-PCR and Western Blot assay. Together, these data again confirmed the nerve tissue specificity of the fish SNAP-25 gene expression.
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| 关 键 词： | SNAP-25基因 海洋硬骨鱼类 生活特征 基因表达 |
| 收稿时间： | 20 February 2006 |
| 修稿时间： | 2006-02-20 |
Characterization of SNAP-25 gene from marine teleostean, <Emphasis Type="Italic">Lateolabrax japonicus</Emphasis> |
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| Chen?Kui,Huang?Xiaohang,Chai?Yingmei,Herbert?Y.?Gaisano.Characterization of SNAP-25 gene from marine teleostean, Lateolabrax japonicus[J].Chinese Journal of Oceanology and Limnology,2007,25(4):378-385. |
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| Authors: | Chen Kui Huang Xiaohang Chai Yingmei Herbert Y Gaisano |
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| Institution: | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China;Graduate University, Chinese Academy of Sciences, Beijing 100039, China;Department of Physiology, University of Toronto, Toronto, M5S IA8, Canada |
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| Abstract: | The t-SNARE protein SNAP-25 (synaptosome-associated protein of 25 kDa) plays an essential role in regulating fusion between
the vesicle and plasma membranes during exocytosis. To clone and characterize SNAP-25 gene, the first step in the functional
study of SNARE proteins in marine teleostean, was to obtain the cDNA of sea perch SNAP-25 (SPsn25) by RT-PCR and RACE-PCR
amplification of a Japanese sea perch. The full-length cDNA of 831bp contains a CDS of 615 bp, coding 204 amino acid residues,
and a 5 UTR of 219bp. Bioinformatic analysis revealed that SPsn25 corresponds with SNAP-25a isoform and shares 91.1% identity
with SNAP-25a of a goldfish and a zebrafish. The SPsn25 expression in both mRNA and protein levels in the Japanese sea perch
had been identified through semi-quantitative RT-PCR and Western Blot assay. Together, these data again confirmed the nerve
tissue specificity of the fish SNAP-25 gene expression.
Supported by the NSFC (No.40476060) and Hi-Tech Research and Development Program of China (No. 2002AA629120). |
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| Keywords: | Lateolabrax japonicus SNARE SNAP-25 |
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