| 蛋白质三级拓扑结构对热稳定性贡献的初步探讨 |
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| 引用本文： | 王霁,张士璀,陈玲玲.蛋白质三级拓扑结构对热稳定性贡献的初步探讨[J].中国海洋大学学报(自然科学版),2009,39(5). |
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| 作者姓名： | 王霁 张士璀 陈玲玲 |
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| 作者单位： | 1. 中国海洋大学海洋生命学院,山东,青岛,266003
2. 山东理工大学生命科学学院,山东,淄博,255049 |
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| 基金项目： | 国家自然科学基金项目(30570383)资助 |
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| 摘 要： | 搜集来自数据库和文献的127个已测定熔解温度和PDB结构的热稳定蛋白,计算了每个蛋白PDB结构的9种拓扑参数,并对各个参数与其自身熔解温度做了线性相关性分析。现有数据结果表明只有CC这个参数与Tm的相关性系数达到了0.33。CC参数表征蛋白结构作为天然接触的网络来处理时的簇系数,因此这一关系表明组成蛋白的基团接触的越紧密,蛋白的热稳定性就越高,这与之前的研究结论相一致。而其它的参数没有发现与Tm有显著的关系。作者从拓扑结构这个方面进行研究为蛋白质的热适应机理研究提供了新的思路。
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| 关 键 词： | 蛋白质 三级结构 拓扑参数 熔解温度 热稳定性 |
Preliminary Study on the Contribution of Protein Structure Topology to Thermostability |
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| WANG Ji,ZHANG Shi-Cui,CHEN Ling-Ling.Preliminary Study on the Contribution of Protein Structure Topology to Thermostability[J].Periodical of Ocean University of China,2009,39(5). |
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| Authors: | WANG Ji ZHANG Shi-Cui CHEN Ling-Ling |
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| Institution: | 1.College of Marine Life Sciences;Ocean University of China;Qingdao 266003;China;2.School of Life Sciences;Shandong University of Technology;Zibo 255049;China |
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| Abstract: | In the present work,nine topological parameters for 127 proteins with 3D structures and melting temperatures(Tm),collected from databases and the literature,were calculated.Then the linear relationship between each parameter and Tm was analyzed.Topological parameter "CC" is defined as the clustering coefficient by treating protein structure as a network of native contacts.It was observed that the correlation coefficient between topological parameter "CC" and Tm is as high as 0.33,indicating that proteins di... |
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| Keywords: | protein tertiary structure topology parameter melting temperature thermostability |
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