| Screening of Extraction Methods for Glycoproteins from Jellyfish （Rhopilema esculentum） Oral-Arms by High Performance Liquid Chromatography |
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| 引用本文： | REN Guoyan,,LI Bafang,*,ZHAO Xue,ZHUANG Yongliang,YAN Mingyan,HOU Hu,ZHANG Xiukun,, CHEN Li Food , Bioengineering Department,Henan University of Science , Technology,Luoyang ,P. R. China College of Food Science , Engineering,Ocean University of China,Qingdao ,P. R. China.Screening of Extraction Methods for Glycoproteins from Jellyfish （Rhopilema esculentum） Oral-Arms by High Performance Liquid Chromatography[J].中国海洋大学学报(英文版),2009,8(1). |
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| 作者姓名： | REN Guoyan LI Bafang * ZHAO Xue ZHUANG Yongliang YAN Mingyan HOU Hu ZHANG Xiukun CHEN Li Food Bioengineering |
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| 作者单位： | REN Guoyan(Food and Bioengineering Department, Henan University of Science and Technology, Luoyang 471003, P. R China;College of Food Science and Engineering, Ocean University of China, Qingdao 266003, P. R. China);LI Bafang,ZHAO Xue,ZHUANG Yongliang,YAN Mingyan,HOU Hu,ZHANG Xiukun,CHEN Li(College of Food Science and Engineering, Ocean University of China, Qingdao 266003, P. R. China)
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| 基金项目： | support from the National High Technology Research and Development Program of China (No.2006AA09Z438) |
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| 摘 要： | In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oralarms, a high performance liquid chromatography (HPLC)–assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction...
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| 关 键 词： | HPLC 高性能液体色谱 糖蛋白 萃取法 水母 |
Screening of Extraction Methods for Glycoproteins from Jellyfish (Rhopilema esculentum) Oral-Arms by High Performance Liquid Chromatography |
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| REN Guoyan,LI Bafang,ZHAO Xue,ZHUANG Yongliang,YAN Mingyan,HOU Hu,ZHANG Xiukun,CHEN Li.Screening of Extraction Methods for Glycoproteins from Jellyfish (Rhopilema esculentum) Oral-Arms by High Performance Liquid Chromatography[J].Journal of Ocean University of China,2009,8(1). |
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| Authors: | REN Guoyan LI Bafang ZHAO Xue ZHUANG Yongliang YAN Mingyan HOU Hu ZHANG Xiukun CHEN Li |
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| Institution: | 1. Food and Bioengineering Department, Henan University of Science and Technology, Luoyang 471003, P. R China;College of Food Science and Engineering, Ocean University of China, Qingdao 266003, P. R. China
2. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, P. R. China |
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| Abstract: | In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotcin. The results showed that the calibration curves for peak area plotted against con-centration for TGP were linear (r=0.9984, y=4.5895x+47.601) over concentrations ranging from 50 to 400mgL-1. The mean extrac-tion recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish (R. esculentum) oral-arms bv four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water ex-traction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophtlized extract was dissolved in Milli-Q water and ana-lyzed directly on a short TSK-GEL G4000PWXL (7.8mm×300mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.Kay words HPLC; glycoprotein; jellyfish; extraction method |
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| Keywords: | HPLC glycoprotein jellyfish extraction method |
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