壳聚糖的脱乙酰度、分子质量和性状对其体外促凝血活性的影响

制备不同脱乙酰度、分子质量的壳聚糖固体粉末和溶液,并利用试管法比较了不同浓度、不同性质及不同状态的样品的体外凝血时间.结果表明:当分子质量相近时,壳聚糖溶液的体外凝血时间随脱乙酰度的增加而减少,脱乙酰度中等的固体粉末具有最强的促凝血能力;当脱乙酰度相近时,只有高分子质量的壳聚糖溶液具有一定的促凝血活性,但固体粉末状态的三种壳聚糖均具有一定的缩短体外凝血时间的能力;同等剂量的壳聚糖溶液和粉末,除了高脱乙酰度、高分子质量的壳聚糖外,其余样品的固体粉末对于缩短体外凝血时间更为有效

The effects of degrees of deacetylation, molecular weights and states of chitosans on procoagulant activities in vitro

To investigate the effects of degrees of deacetylation, molecular weights and states (solutions or solid powder) of chitosans on the procoagulant activities in vitro, the blood clotting time of the samples with different concentrations, properties and states were tested the in tube method. When molecular weights were close, the blood clotting time of chitosan solutions decreased with the increasing of degree of deacetylation, however, the procoaglant ability of the chitosan powder with medium degree of deacetylation was the best of all. When degrees of deacetylation were close, the solution of chitosan with high molecular weight was the only one that had procoagulant activity, but all the powders could decrease the clotting time in vitro. All solid chitosan powders, except the one with high degree of deacetylation and molecular weight, promoted the blood coaglations more efficiently than the correspondent solution at the same concentration.