| 军曹鱼群体遗传结构的AFLP分析 |
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| 引用本文： | 王中铎,史沛鑫,苏惠娜,赵洁,沈恩健,刘楚吾. 军曹鱼群体遗传结构的AFLP分析[J]. 广东海洋大学学报, 2011, 31(1): 12-17 |
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| 作者姓名： | 王中铎 史沛鑫 苏惠娜 赵洁 沈恩健 刘楚吾 |
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| 作者单位： | 广东海洋大学水产学院,南海水产经济动物增养殖广东普通高校重点实验室,广东湛江524025 |
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| 基金项目： | “十一五”国家科技支撑计划(2007BAD29B03); 广东省自然科学基金(8452408801001272) |
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| 摘 要： | 筛选出10对扩增片段长度多态分析技术(Amplified fragment length polymorphism,AFLP)的选择性扩增引物(E-aga/M-cgt、E-aga/M-cga、E-agc/M-cga、E-agg/M-cga、E-act/M-cgc、E-aag/M-cgc、E-aca/M-cga、E-aac/M-cgc、E-aac/M-cac和E-aac/M-cat),分析了4个军曹鱼(Rachycentron canadum)全人工繁育群体海南1(HN1)、海南2(HN2)、湛江(ZJ)和流沙湾(LS)的遗传结构。结果表明:总体多态位点比例(83.9%)、分化指数(0.427 8)和Shannon信息指数(0.614 9)均处于较高水平,遗传多样性丰富;主坐标分析和邻位连接树中LS-HN2支与ZJ-HN1支明显分离,各群体内多样性水平存在差异(遗传多样性大小依次为HN1、HN2、LS、ZJ),但总体基因流仍然较大(Nm=10.327),分子方差分析(AMOVA)获得的群内方差(53%)也略大于群间(47%)。
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| 关 键 词： | 军曹鱼 群体结构 遗传多样性 扩增片段长度多态分析技术(AFLP) |
Analysis on Population Genetic Structure of Cobia (Rachycentron canadum) with AFLP Markers |
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| WANG Zhong-duo,SHI Pei-xin,SU Hui-na,ZHAO Jie,SHEN En-jian,LIU Chu-wu. Analysis on Population Genetic Structure of Cobia (Rachycentron canadum) with AFLP Markers[J]. Journal of Zhanjiang Ocean University, 2011, 31(1): 12-17 |
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| Authors: | WANG Zhong-duo SHI Pei-xin SU Hui-na ZHAO Jie SHEN En-jian LIU Chu-wu |
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| Affiliation: | WANG Zhong-duo,SHI Pei-xin,SU Hui-na,ZHAO Jie,SHEN En-jian,LIU Chu-wu(Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes,Fisheries College,Guangdong Ocean University,Zhanjiang 524025,China) |
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| Abstract: | Ten primer pairs of AFLP,E-aga/M-cgt,E-aga/M-cga,E-agc/M-cga,E-agg/M-cga,E-act/M-cgc,E-aag/M-cgc,E-aca/M-cga,E-aac/M-cgc,E-aac/M-cac and E-aac/M-cat,were selected to determine genetic structure of 4 cultured cobia(Rachycentron canadum) populations(Hainan1,Hainan2,Liushawan and Zhanjiang,and 30 individuals for each) based on 0/1 mat rix of 500 loci.Total percentage of polymorphic loci(83.9%),Nei's(1973) gene diversity(0.4278) and Shannon diversity index(0.6149) showed high values and genetic diversity.Differ... |
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| Keywords: | cobia(Rachycentron canadum Linnaeus) AFLP population structure genetic diversity |
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