| 眼柄粗提物对凡纳滨对虾性分化的影响 |
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| 引用本文： | 朱春华,赵光凤,李广丽. 眼柄粗提物对凡纳滨对虾性分化的影响[J]. 广东海洋大学学报, 2011, 31(1) |
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| 作者姓名： | 朱春华 赵光凤 李广丽 |
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| 作者单位： | 广东海洋大学水产学院,广东湛江,524025 |
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| 基金项目： | 广东省自然科学基金(8152408801000024); 广东省科技攻关项目(2008B020800006) |
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| 摘 要： | 采用生化方法制备凡纳滨对虾[Litopenaeus(Penaeus)vannamei)]眼柄粗提物,用活体注射法研究不同粗提物剂量对去眼柄凡纳滨对虾卵母细胞直径大小的影响,以检验其性腺抑制素(Gonadinhibiting hormone,GIH)的生物活性;在凡纳滨对虾仔虾后17、22和27 d,分别以不同浓度眼柄粗提物浸泡24 h,结果显示:除0.001个眼柄/mL的GIH浓度外,0.01、0.1和1个眼柄/mL组均显著降低凡纳滨对虾卵母细胞直径(P﹤0.05);浸泡处理后,仔虾后17 d GIH处理组凡纳滨对虾雌性率达70.1%～80.1%;仔虾后22 d GIH处理组雌性率达65.8%～71.7%,各浓度GIH处理组雌性率比对照组(雌雄比例约为1:1)显著提高(P﹤0.05);仔虾后27 d,0.01和1个眼柄/mL处理组雌性率(约58%)略高于对照组,但0.1个眼柄/mL处理组雌性率(73.1%)与对照组相比显著提高(P﹤0.05)。结果表明较低浓度的眼柄粗提物可明显诱导凡纳滨对虾雌性化,在仔虾后22 d前处理效果最佳。
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| 关 键 词： | 凡纳滨对虾 眼柄粗提物 性分化 性腺抑制素(GIH) 雄性腺激素(AGH) |
Effects of Eyestalk Extracts on the Sex Differentiation on Litopenaeus vannamei |
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| ZHU Chun-hua,ZHAO Guang-feng,LI Guang-li. Effects of Eyestalk Extracts on the Sex Differentiation on Litopenaeus vannamei[J]. Journal of Zhanjiang Ocean University, 2011, 31(1) |
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| Authors: | ZHU Chun-hua ZHAO Guang-feng LI Guang-li |
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| Affiliation: | ZHU Chun-hua,ZHAO Guang-feng,LI Guang-li(Fisheries College,Guangdong Ocean University,Zhanjiang 524025,China) |
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| Abstract: | The eyestalk extracts were obtained by biochemistry methods and their activities of gonad-inhibiting hormone(GIH) were checked by vivo injection(0.001,0.01,0.1,and 1 eyestalk per mL) on female Litopenaeus vannamei.The shrimps were immersed in different doses of eyestalk extracts(0.01,0.1 for 24 hours,and 1 eyestalk per mL) at 17,22 and 27 days after postlarva,respectively,and the effects of GIH on the sex differentiation were investigated.The results showed that strong GIH activities existed in the eyestalk... |
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| Keywords: | Litopenaeus vannamei eyestalk extracts sex differentiation gonad-inhibiting hormone(GIH) androgenic gland hormone(AGH) |
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