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24-表油菜素内酯对浒苔(Ulva prolifera)内源植物激素及相关生理影响
引用本文:胡 伟,孙 雪,范美华,高秀秀,徐年军.24-表油菜素内酯对浒苔(Ulva prolifera)内源植物激素及相关生理影响[J].海洋与湖沼,2014,45(5):1071-1077.
作者姓名:胡 伟  孙 雪  范美华  高秀秀  徐年军
作者单位:宁波大学 教育部应用海洋生物技术重点实验室 宁波 315211;浙江省海洋生物工程重点实验室 宁波 315211;宁波大学 教育部应用海洋生物技术重点实验室 宁波 315211;浙江省海洋生物工程重点实验室 宁波 315211;宁波大学 教育部应用海洋生物技术重点实验室 宁波 315211;浙江省海洋生物工程重点实验室 宁波 315211;宁波大学 教育部应用海洋生物技术重点实验室 宁波 315211;浙江省海洋生物工程重点实验室 宁波 315211;宁波大学 教育部应用海洋生物技术重点实验室 宁波 315211;浙江省海洋生物工程重点实验室 宁波 315211
基金项目:国家自然科学基金项目, 41276122 号; 国家教育部博士点基金, 20123305110002 号
摘    要:采用植物激素的微量LC-MS检测等方法,研究了24-表油菜素内酯(EBR)对宁波浒苔(Ulva prolifera)内源植物激素含量及其叶绿素荧光参数、可溶性蛋白和糖含量、抗氧化酶活性等的影响。结果表明,浒苔体内含有吲哚乙酸、赤霉素、玉米素、反式玉米素核苷、异戊烯腺嘌呤核苷、茉莉酸等多种促生长性内源植物激素,但不含有异戊烯腺嘌呤。内源植物激素对24-表油菜素内酯的响应程度不同,大多数植物激素在处理第1天含量变化最快。低浓度24-表油菜素内酯处理时,各种内源植物激素的含量增高,均呈现先升高后降低的趋势。0.2 mg/L 24-表油菜素内酯处理组的效果最明显。在该浓度下,24-表油菜素内酯对生长相关激素含量、最大光能转化效率(Fv/Fm)和相对电子传递速率(ETR)、可溶性蛋白和可溶性糖含量、POD活性等均有较大的促进作用;在0.1 mg/L 24-表油菜素内酯处理组中,浒苔的体外抗氧化能力最强。

关 键 词:浒苔  植物激素  -表油菜素内酯  生理反应
收稿时间:2013/12/26 0:00:00
修稿时间:3/5/2014 12:00:00 AM

EFFECTS OF 24-EPIBRASSINOLIDE ON PHYTOHORMONE VARIATION AND RELATED PHYSIOLOGICAL RESPONSE OF ULVA PROLIFERA
HU Wei,SUN Xue,FAN Mei-Hu,GAO Xiu-Xiu and XU Nian-Jun.EFFECTS OF 24-EPIBRASSINOLIDE ON PHYTOHORMONE VARIATION AND RELATED PHYSIOLOGICAL RESPONSE OF ULVA PROLIFERA[J].Oceanologia Et Limnologia Sinica,2014,45(5):1071-1077.
Authors:HU Wei  SUN Xue  FAN Mei-Hu  GAO Xiu-Xiu and XU Nian-Jun
Institution:Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo 315211, China;Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo 315211, China;Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo 315211, China;Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo 315211, China;Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo 315211, China
Abstract:race LC-MS method was applied to determine algal phytohormone, and the effects of 24-epibrassinolide (EBR) on endogenous phytohormone content, chlorophyll fluorescence parameters, soluble protein and carbohydrate contents, and antioxidant capacity of macroalgae Ulva prolifera in Ningbo, China were investigated. Results revealed many types of phytohormones such as IAA, GA, ZR, TRZ, iPA, and JA but iP. The phytohormones showed different sensitivity to EBR, and most of them responded on the first day of treatment. Under low concentration of EBR treatment, all endogenous phytohormone contents in U. prolifera increased, showing a tendency of first increase then decrease. The 0.2 mg/L EBR group showed the best effect of promoting phytohormone, Fv/Fm (indicative of photochemical efficiency), ETR, soluble protein and soluble sugar contents, and POD of U. prolifera. However, the strongest in-vitro antioxidative capacity was detected in the 0.1 mg/L EBR group.
Keywords:Ulva prolifera    phytohormones    24-epibrassinolide    physiological response
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