| 米曲霉中性蛋白酶特性的研究 |
| |
| 引用本文： | 杨世平,邱德全. 米曲霉中性蛋白酶特性的研究[J]. 广东海洋大学学报, 2006, 26(4): 22-25 |
| |
| 作者姓名： | 杨世平 邱德全 |
| |
| 作者单位： | 广东海洋大学广东省海洋开发研究中心,广东,湛江,524025 |
| |
| 基金项目： | 广东省湛江市科技局科研项目 |
| |
| 摘 要： | 研究了米曲霉中性蛋白酶的特性。结果表明,米曲霉中性蛋白酶的最佳提取方法为加入pH 7.2的缓冲液研匀,置恒温(30℃)培养箱中提取30 min,每10 min搅动1次,最后用4层纱布过滤。酶反应最适温度为55℃,最适pH值为7.2。NaCl和EDTA对其有一定的抑制作用。酶的热稳定性好,在45℃的温度下处理24 h后,酶活力仍有45.5%。米曲霉中性蛋白酶与培养物一起保存时,稳定性也较好,在4℃保存一个月后,酶活力几乎无变化。
|
| 关 键 词： | 米曲霉 中性蛋白酶 提取方法 酶活力 |
| 文章编号： | 1007-7995(2006)04-0022-04 |
| 修稿时间： | 2005-11-21 |
Study on Characters of Neutral Protease from Aspergillus or yzae |
| |
| YANG Shi-ping,QIU De-quan. Study on Characters of Neutral Protease from Aspergillus or yzae[J]. Journal of Zhanjiang Ocean University, 2006, 26(4): 22-25 |
| |
| Authors: | YANG Shi-ping QIU De-quan |
| |
| Abstract: | The characters of neutral protease from Aspergillus oryzae were studied.The results show that the suitable extraction conditions of neutral protease are under 30℃,pH 7.2,30 min.The extraction is stirred per 10 min,finally filtrated with four layers of gauze.The optimal pH and te mperature for enzyme activity are 7.2 and 55℃.Sodium chloride(NaCl) and EDTA inhibit the enzyme.The enzyme is stable,which retains 45.5% of its original activity after being treated under 45℃ for 24 h.The enzyme is also stable wh ile preserved with medium,for its activity is almost unchangeable after having been preserved under 4℃ for a month. |
| |
| Keywords: | Aspergillus oryzae neutral protease extration co ndition enzyme activity |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
