Degradation ofn-paraffin mixture by marine microorganisms in enriched seawater medium

The ability to degraden-paraffin mixture of two bacterial strains,Caulobacter sp. andFlavobacterium sp., isolated from sea water of Tokyo Bay was studied experimentally in the enriched seawater (ESW) medium. These bacteria degraded actively the mixture ofn-tridecane,n-tetradecane,n-pentadecane andn-hexadecane. The maximum rate of degradation was observed after a lag period of 2 to 8 day and these bacteria were found to degrade then-paraffin mixture at rates calculated to be in a range from 3.3×10^?12 to 3.4×10^?11 mg-oil cell^?1 h^?1 at 20°C. The maximum degradation rate,r _m mg-oil l^?1 h^?1, was correlated with the amount of the initial totaln-paraffin,S mg-oil l^?1, as expressed by the following equation: $$rm = (rm)\max \left( {\frac{S}{{S + Km}}} \right)$$ where (r _m )_max denotes the largest value ofr _m whenn-paraffin exists in large excess andK _m is a constant and represents the amount ofn-paraffin at which the degradation rate,r _m , reaches 1/2 of its largest value, (r _m )_max. The values of (r _m )_max andK _m were calculated to be as follows: In the case ofCaulobacter sp. (strain KM-1), (r _m )_max=6.0 mg-oil l^?1 h^?1 andK _m =191 mg-oillesw ^?1; in the case ofFlavobacterium sp., (r _m )_max=5.47 mg-oil l^?1 h^?1 andK _m =152 mg-oillesw ^?1.