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Distribution and expression in vitro and in vivo of DNA vaccine against lymphocystis disease virus in Japanese flounder(Paralichthys olivaceus) |
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| Authors: | ZHENG Fengrong SUN Xiuqin LIU Hongzhan WU Xingan ZHONG Nan WANG Bo ZHOU Guodong First Institute of Oceanography SOA Qingdao China Marine College Sh ong University at Weihai Weihai China |
| Institution: | ZHENG Fengrong,SUN Xiuqin,LIU Hongzhan,WU Xingan,ZHONG Nan,WANG Bo,ZHOU Guodong First Institute of Oceanography SOA,Qingdao 266061,China. Marine College,Sh,ong University at Weihai,Weihai 264209,China Department of Microbiology,Fourth Military Medical University,Xi'an 710032,China Hainan Green Food Industrial company,Ltd,Haikou 571127,China |
| Abstract: | Lymphocystis disease,caused by the lymphocystis disease virus (LCDV),is a significant worldwide problem in fish industry causing substantial economic losses.In this study,we aimed to develop the DNA vaccine against LCDV,using DNA vaccination technology.We evaluated plasmid pEGFP-N2-LCDV1.3 kb as a DNA vaccine candidate.The plasmid DNA was transiently expressed after liposome transfection into the eukaryotic COS 7 cell line.The distribution and expression of the DNA vaccine (pEGFP-N2-LCDV1.3kb) were also ana... |
| Keywords: | DNA vaccine lymphocystis disease virus Paralichthys olivaceus |
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