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利用微卫星标记估计凡纳滨对虾生长性状的遗传力
引用本文:安迪,刘小林,黄浩.利用微卫星标记估计凡纳滨对虾生长性状的遗传力[J].海洋学报(英文版),2015,34(6):59-65.
作者姓名:安迪  刘小林  黄浩
作者单位:西北农林科技大学动物科技学院, 陕西省农业分子生物学重点实验室, 陕西杨凌, 712100,西北农林科技大学动物科技学院, 陕西省农业分子生物学重点实验室, 陕西杨凌, 712100,海南广泰海洋育种有限公司, 海南省海口市, 570000
基金项目:The Agricultural Science and Technology Achievement Transformation Fund Project of Ministry of Science and Technology, China under contract No. 2012GB2E200361; the Key Laboratory of Marine Biology, Institute of Oceanlogy, Chinese Academy of Sciences; Qianjiang distinguished experts of Hangzhou; Extension and Demonstration Program in Desalting Culture of Litopenaeus Vannamei New Breed “Ke Hai 1”, Northwest A&F University, China under contract No. XNY2013-4.
摘    要:Pacific whiteleg shrimp(Litopenaeus vannamei) is an economically relevant shrimp species in many Asian countries. The specific objective of the current research was to assess microsatellite markers in screening the fastgrowth of domesticated L. vannamei stocks to establish a founder population for breeding-selection plans. The postlarvae produced by the reproduction of second generation broodstock were cultured in the same conditions throughout a five months growing period. Ninety juvenile shrimp were selected from the slow-, medium- and the fast-growth groups, and ten microsatellite markers were used to investigate their genetic diversity, and to understand the improvement of a breeding-selection scheme. Ten polymorphic loci(markers)(M1–M10) were produced at ten loci in this sample, among them Primer M8 was the highest polymorphic locus and M7 was the lowest one. A specific locus was found in the fast-growth group using Primer M5. The longest genetic distance(0.481) was determined between the fast- and medium-growth groups and the shortest(0.098) was between the slow- and medium-growth groups; therefore, the largest genetic identity(0.946) was observed between the slowand medium-growth groups and the smallest(0.667) was observed between the medium- and fast-growth groups.The Unweighted Paired Group with Arithmetic Average(UPGMA) dendrogram based on Nei's genetic distances provided two different groups; the first consist of the slow- and medium-growth groups and the second the fastgrowth group. Selection response and realized heritability for growth were 11.55% and 31.26%, respectively.Therefore, this set of microsatellite markers would provide a useful tool in shrimp breeding schemes.

关 键 词:遗传参数  生长  遗传力  微卫星  分子标记
收稿时间:2014/1/15 0:00:00
修稿时间:2014/5/16 0:00:00

Using microsatellite markers to identify heritability of Pacific whiteleg shrimp Litopenaeus vannamei
ANDRIANTAHINA Farafidy,LIU Xiaolin and HUANG Hao.Using microsatellite markers to identify heritability of Pacific whiteleg shrimp Litopenaeus vannamei[J].Acta Oceanologica Sinica,2015,34(6):59-65.
Authors:ANDRIANTAHINA Farafidy  LIU Xiaolin and HUANG Hao
Institution:1.College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China2.Hainan Guangtai Ocean Breeding Company Limited, Haikou 570000, China
Abstract:Pacific whiteleg shrimp (Litopenaeus vannamei) is an economically relevant shrimp species in many Asian countries. The specific objective of the current research was to assess microsatellite markers in screening the fastgrowth of domesticated L. vannamei stocks to establish a founder population for breeding-selection plans. The postlarvae produced by the reproduction of second generation broodstock were cultured in the same conditions throughout a five months growing period. Ninety juvenile shrimp were selected from the slow-, medium- and the fast-growth groups, and ten microsatellite markers were used to investigate their genetic diversity, and to understand the improvement of a breeding-selection scheme. Ten polymorphic loci (markers) (M1-M10) were produced at ten loci in this sample, among them Primer M8 was the highest polymorphic locus and M7 was the lowest one. A specific locus was found in the fast-growth group using Primer M5. The longest genetic distance (0.481) was determined between the fast- and medium-growth groups and the shortest (0.098) was between the slow- and medium-growth groups; therefore, the largest genetic identity (0.946) was observed between the slowand medium-growth groups and the smallest (0.667) was observed between the medium- and fast-growth groups. The Unweighted Paired Group with Arithmetic Average (UPGMA) dendrogram based on Nei's genetic distances provided two different groups; the first consist of the slow- and medium-growth groups and the second the fastgrowth group. Selection response and realized heritability for growth were 11.55% and 31.26%, respectively. Therefore, this set of microsatellite markers would provide a useful tool in shrimp breeding schemes.
Keywords:genetic parameters  growth  heritability  microsatellite  molecular markers
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