| Affiliation: | 1. The State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, P.R. China
2. College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P.R. China;The State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, P.R. China |
| Abstract: | Yeast strain Y68 producing high level of pullulan was isolated from the phyton collected in Toulouse, France. This strain was identified to be Rhodotorula bacarum by BIOLOG analysis. This is the first report that pullulan was produced by Rhodotorula bacarum. The optimal medium (g L−1) for pullulan production by this strain was 80 glucose, 20 soybean cake hydrolysate, 5 K2HPO4, 1 NaCl, 0.2 MgSO4·7H2O, 0.6 (NH4)2SO4, pH 7.0. Under this condition, 54 gL−1 pullulan was produced within 60 h at 30°C. Pullulan is a better starting material for producing marine prodrugs. |
