中国南海软珊瑚真菌Eupenicillium sp. DX-SER3 (KC871024)的次级代谢产物研究

文章旨在对中国南海软珊瑚来源真菌Eupenicillium sp. DX-SER3 (KC871024)进行次级代谢产物的研究。利用硅胶层析柱、正反相中压层析柱以及半制备高效液相等方法对该菌株的大米发酵产物进行分离纯化, 利用核磁共振、质谱等波谱学方法并参考文献数据对分离的单体化合物进行结构鉴定。从中分离得到5个已知化合物: 烟曲霉酸、β-腺苷、2'-脱氧胸腺嘧啶核苷、N-acyltryptamine和对羟基苯甲醛。其中, 烟曲霉酸在该菌株中产量很高, 预示着该菌株具有开发成该类化合物的工程菌株的潜力。文章还探讨了烟曲霉酸抗植物病原真菌的潜力, 发现效果并不明显。

Study on the secondary metabolites from the South China Sea soft coral-derived fungus Eupenicillium sp. DX-SER3 (KC871024)

We aim to study the secondary metabolites from the South China Sea soft coral-derived fungus Eupenicillium sp. DX-SER3 (KC871024). The rice fermentation products of the strain were purified by comprehensive chromatography methods of silica gel column, medium pressure preparative liquid chromatography (MPLC), ostade-cylsilane (ODS), and semi- preparative HPLC. The compounds’ structures were identified by nuclear magnetic resonance spectroscopy, mass spectroscopy, and comparison with the reported data. Five known compounds were obtained and identified as helvolic acid, β-adenosine, 2'-deoxythymidine, N-acyltryptamine, and p-hydroxybenzaldehyde. The high yield of the helvolic acid indicates that this strain has the potential to develop an engineered strain of this kind of compound. Helvolic acid was also tested for the activities of phytopathogenic fungus, and the result was not clear.