东太平洋海洋微生物种群多样性初步研究

以东太平洋海洋微生物群落为研究对象,用稀释平板分离法,从海水中分离得到67株细菌。在形态观察的基础上选取48株进行培养,然后进行16S rDNA基因扩增,并用限制性内切酶RsaI和MspI对PCR产物进行ARDRA(Amplified rDNA restriction analysis)多态性分析,共得到10种不同的操作分类单元(Operational Taxonomic Unit,OTU)。其中OTU4和OTU10所包含的菌株分别占总分离物的35.4%和18.8%,为优势分离菌。优势分离菌的ERIC-PCR基因组指纹图分析表明,前者的17株分离物共有10种不同的指纹图类型,而后者的9株分离物有3种。结果显示,东太平洋海域的海水和底质沉积物具有明显的微生物种群多样性特征。

A PRIMARY STUDY ON POPULATION BIODIVERSITY OF MARINE MICROORGANISMS FROM EAST PACIFIC

Marine bacteria from the samples of sea sediments and seawater were directly plated on isolation media and the biodiversity of isolates was examined with DNA fingerprinting.48 single colonies were obtained from the media.ARDRA with enzyme RsaI and MspI revealed 10 operational taxonomic units(OTU) which were dominated by OTU4 group which accounts for 17 isolates and OTU10 group which accounts for 9 isolates.The biodiversity of isolates from these two dominant OTU groups was further investigated by a genomic fingerprinting technique,ERIC-PCR.The results indicated that there were 10 different ERIC-PCR types present among the OTU4 group while only 3 among the OTU10.The data indicated rich diversity profiles of marine microorganisms were presented in the East Pacific.