浒苔Up-MIR-843及其靶基因分析

非编码小分子RNA (non-coding small RNAs, microRNAs)在植物的生长，发育和应对非生物胁迫过程中发挥着重要作用。本研究分析了氮剥夺和高温刺激条件下，浒苔Up-miR-843及其靶基因的表达变化。结果表明，生物信息学分析可以得到184个Up-miR-843靶基因。氮缺乏可以显著增加Up-miR-843的水平，同时降低细胞周期相关基因Cyclin A3和Cyclin L的表达，而高温刺激对Up-miR-843的表达不产生影响。同时，Up-miR-843高表达的浒苔与正常条件培养的浒苔比较，生物量和光合作用合成速率显著增加。因此，我们推测氮剥夺和高温应激响应microRNAs可能通过介导其靶基因表达，调节浒苔的生长。

Expression analyses of miRNA Up-MIR-843 and its target genes in Ulva prolifera

microRNAs (miRNA) families play a critical role in plant growth, development, and responses to abiotic stress. In this study, we characterized Up-miR-843 and its targets genes in Ulva prolifera responses to nitrogen depravation and heat stress. The data demonstrated that 184 target genes of Up-miR-843 could be successfully validated. N deficiency not heat stress stimulus induced increase in abundance of the Up-miR-843 while exhibited reverse expression of target genes, including cyclin A3 and cyclin L, which were strictly required for cell cycle progression. In addition, U. prolifera with highly expression of Up-miR-843 showed improved biomass, and photosynthesis compared with that under normal growth conditions. Thus, the N deprivation and heat responsive miRNAs might be a possible member mediating the expression of these target genes, which further regulated the growth of U. prolifera.