抗冻蛋白基因重组质粒的转化与扩增

鲽鱼(Pseudopleuronectus americanus)为了适应冬季低溫的海水,在肝脏中产生一种抗冻蛋白释放到血液中。当血液中含有这种抗冻蛋白时,即使在-1.5°C的溫度下,血液仍不会凝固结冰。据报道,这是一种小分子的、富含丙氨酸的蛋白,丙氨酸占氨基酸

TRANSFORMATION AND AMPLIFICATION OF RECOMBINANT PLASMID CONTAINING ANTIFREEZE PROTEIN GENES

Some of the plasmid containing antifreeze protein genes were assayed by agarose gel elec-trcphoresis and others transformed into the E.coli C₆₀₀. It was amplificated in the BL-med-iam and then purified by speed-extraction method. The plasmid DNA extracted was charact-eristed by agarose gel eleetphoresis again, showing that the plasmid containing antifreeze protein genes has been transformed into the E.coli C₆₀₀ and amplified.