抗冻蛋白基因重组质粒的转化与扩增 |
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引用本文: | 徐权汉,钱 标.抗冻蛋白基因重组质粒的转化与扩增[J].海洋科学,1985,9(3):45-47. |
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作者姓名: | 徐权汉 钱 标 |
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作者单位: | 中国科学院海洋研究所
(徐权汉),中国科学院生物化学研究所(钱标) |
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摘 要: | 鲽鱼(Pseudopleuronectus americanus)为了适应冬季低溫的海水,在肝脏中产生一种抗冻蛋白释放到血液中。当血液中含有这种抗冻蛋白时,即使在-1.5°C的溫度下,血液仍不会凝固结冰。据报道,这是一种小分子的、富含丙氨酸的蛋白,丙氨酸占氨基酸
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TRANSFORMATION AND AMPLIFICATION OF RECOMBINANT PLASMID CONTAINING ANTIFREEZE PROTEIN GENES |
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Abstract: | Some of the plasmid containing antifreeze protein genes were assayed by agarose gel elec-trcphoresis and others transformed into the E.coli C600. It was amplificated in the BL-med-iam and then purified by speed-extraction method. The plasmid DNA extracted was charact-eristed by agarose gel eleetphoresis again, showing that the plasmid containing antifreeze protein genes has been transformed into the E.coli C600 and amplified. |
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