青鳞鱼下脚料水解蛋白的制取及其营养评价

以青鳞鱼（Harengula zunast)下脚料为原料，采用碱萃取，蛋白酶水解，浓缩，喷雾干燥等手段，制得水解鱼蛋白。研究了碱萃取，蛋白酶水解条件及不同沉淀方法对水解鱼蛋白沉淀效果的影响，并对水解鱼蛋白制品营养价值进行了评价，结果表明，下脚料水解鱼蛋白制品含粗蛋白72．9％，氨基酸总量67．6％，其中必需氨基酸占44．6％，氨基酸分为96，苏氨酸为其限制性氨基酸（1973年FAO／WHO标准），无机盐钙，镁，铁，锌，铜，磷含量丰富，以有机溶剂（异丙醇，乙醇)为沉淀剂沉淀水解蛋白时，粗蛋白损失率约为30％（干基计）。

PROCESSING OF HYDROLYZED PROTEIN FROM Harengula zunast's OFFAL AND ITS NUTRITIONAL EVALUATION

Hydrolyzed protein was obtained from Harengula zunast's offal by alkal;extraetion, enzymatic hydrolysis, concentration and spray dry. The conditions of the alkal;extraction enzymatic hydrolysis and the effects of different sediment methods on hydrolyzed protein were studied.The content of crude protein of hydrolyzed protein is 72.9%. The total amino acid content was 67.6% with the essential amino acids being 44.6%. The amino acid score is 96 with the limited amino acid being Thr as compared with the FAO/WHO suggested level (1973). Hydrolyzed protein is rich in minerals such as Ca, Mg , Fe , Zn , Cu , P. The method of precipitating hydrolyzed protein with organic solvent (isopropyl alcohol, ethanol) makes the loss rate of crude protein of hydrolyzed protein be 30%(dry basis).