Insertal orientation has no influence on the expression ofgfp gene and the growth of the hostSynechococcus sp. PCC7942 |
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| Authors: | Lu Yongzhong Zhang Xuecheng |
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| Affiliation: | 1. College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P .R .China;College of Life Science, Qingdao University of Science and Technology, Qingdao 266003, P .R .China
2. College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P .R .China |
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| Abstract: | In transgenic process, a foreign gene can be integrated in the host genome in two directions, which may influence its expression. In order to study the effects of insertal orientation, the gfp reporter gene was inserted in the isiAB locus of Synechococcus sp. PCC7942 in different directions, and the GFP expression levels and the growth of the transgenic algae were compared. It was showed that the gfp gene could express in each direction, and no significant difference was detected on algal growth and GFP expression levels between the two recombinant algae. |
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| Keywords: | orientation effect homologous recombination gfp gene flow cytometry Synechococcus sp.PCC7942 |
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