A culture-free method for detection of Vibrio vulnificus from coastal seawater based on loop-mediated isothermal amplification targeting vcgC gene |
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Authors: | LI Yongjun ZHENG Zejun ZHAO Yulong WEI Xiaon ZHU Lin and HUANG Xitai |
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Institution: | 1.The College of Life Sciences, Nankai University, Tianjin 300071, China2.The College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China |
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Abstract: | Vibrio vulnificus is an opportunistic pathogen widely distributed in estuarine and coastal seawaters.
In this study, a culture-free method was developed to rapid detection of V. vulnificus in all seasons,
based on loop-mediated isothermal amplification (LAMP) targeting virulent-correlated gene (vcg).
The new assay method allows differentiation between the virulent and non-virulent strain of V.
vulnificus accurately. This method also allows effective detection of the pathogeny in winter when
the bacterium lives in the viable but non-culturable (VBNC) state. A total of 30 costal seawater
samples collected in all seasons were used for the evaluation of this method. The results show that
the method is sensitive, accurate and convenient. |
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Keywords: | Vibrio vulnificus LAMP virulence-correlated gene seawater culture-free VBNC |
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