| 用微卫星分析细鳞鲑(Brachymystax lenok)连续3代选育群体的遗传结构 |
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| 引用本文： | 黄天晴,徐革锋,谷伟,王炳谦,张玉勇,郑先虎,姚作春,赵成,鲁翠云. 用微卫星分析细鳞鲑(Brachymystax lenok)连续3代选育群体的遗传结构[J]. 海洋与湖沼, 2018, 49(4): 858-865 |
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| 作者姓名： | 黄天晴 徐革锋 谷伟 王炳谦 张玉勇 郑先虎 姚作春 赵成 鲁翠云 |
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| 作者单位： | 中国水产科学研究院黑龙江水产研究所 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项，HSY201601号；现代农业产业技术体系专项资金资助，CARS-46号；中国水产科学研究院基本科研业务费，2018HY-ZD0302号；国家水产种质资源平台，2018DKA30470号。 |
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| 摘 要： | 本研究拟利用微卫星标记分析细鳞鲑(Brachymystax lenok)连续3代选育群体的遗传结构及差异。通过筛选出的22对细鳞鲑微卫星引物,利用PCR进行扩增后进行毛细管凝胶电泳,利用Gene Mapper V4.1软件进行图像收集和数据分析。在3代共96个样本中共检测到181个等位基因,各标记检测的等位基因数为2—26个,平均为8.227个;3代平均等位基因数(Na)为6.500—6.773,平均有效等位基因数(Ne)为3.356—3.649,3代间Na和Ne差异均不显著;3代平均观测杂合度(Ho)为0.462—0.530,平均多态信息含量(PIC)为0.459—0.525,平均期望杂合度(He)为0.494—0.566;F2和F3的Ho、He、PIC 3项遗传多样性参数均显著低于F1(P0.05);Hard-Weinberg平衡检验结果表明细鳞鲑3代选育群体整体保持了遗传平衡状态,但经Bonferroni校正后,尚有2个标记在F1和F3极限著偏离遗传平衡(P0.0005),3个标记在F2极限著偏离遗传平衡(P0.0005)。细鳞鲑在选育过程中通过群体选育等方法注重了对稀有等位基因的保护,在细鳞鲑多代选育过程中保持了较高的多态性水平,但在选育过程中某些等位基因出现了富集现象,3代间的遗传分化也较小,仅1.49%的遗传变异来自群体间,表明细鳞鲑群体尚具有持续选育的潜力。
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| 关 键 词： | 细鳞鲑 微卫星标记 遗传结构 |
| 收稿时间： | 2018-01-30 |
| 修稿时间： | 2018-02-23 |
GENETIC STRUCTURE OF THREE CONSECUTIVE BREEDING GENERATIONS OF BRACHYMYSTAX LENOK REVEALED BY MICROSATELLITE MARKERS |
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| HUANG Tian-Qing,XU Ge-Feng,GU Wei,WANG Bing-Qian,ZHANG Yu-Yong,ZHENG Xian-Hu,YAO Zuo-Chun,ZHAO Cheng and LU Cui-Yun. GENETIC STRUCTURE OF THREE CONSECUTIVE BREEDING GENERATIONS OF BRACHYMYSTAX LENOK REVEALED BY MICROSATELLITE MARKERS[J]. Oceanologia Et Limnologia Sinica, 2018, 49(4): 858-865 |
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| Authors: | HUANG Tian-Qing XU Ge-Feng GU Wei WANG Bing-Qian ZHANG Yu-Yong ZHENG Xian-Hu YAO Zuo-Chun ZHAO Cheng LU Cui-Yun |
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| Affiliation: | Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China,Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China,Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China,Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China,Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China,Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China,Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China,Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China and Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China |
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| Abstract: | |
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| Keywords: | Brachymystax lenok microsatellite genetic structure |
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