| 球形棕囊藻对不同氮源的吸收利用机制 |
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| 引用本文： | 蒙蕊,宋秀贤,刘淑雅,姜文彬,俞志明.球形棕囊藻对不同氮源的吸收利用机制[J].海洋与湖沼,2018,49(4):802-808. |
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| 作者姓名： | 蒙蕊 宋秀贤 刘淑雅 姜文彬 俞志明 |
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| 作者单位： | 中国科学院海洋生态与环境科学重点实验室(中国科学院海洋研究所);青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室;中国科学院大学 |
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| 基金项目： | 国家重点研发计划，2017YFC1404300号；鳌山科技创新计划项目“近海生态灾害发生机理与防控策略”，2016ASKJ02-3号；青岛海洋科学与技术国家实验室鳌山人才计划资助，2017ASTCP-OS16号。 |
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| 摘 要： | 本文以球形棕囊藻为实验材料,从营养盐利用及生理生化角度研究了不同氮源对其生长的影响。研究结果表明:球形棕囊藻以尿素为氮源的藻密度以及叶绿素a浓度均高于以NH_4Cl或NaNO_3为氮源的藻密度以及叶绿素a浓度;硝酸盐还原酶(NR)及脲酶(urease)活性表达受培养基中氮源浓度及吸收速率调控,硝酸盐还原酶在以NaNO_3为氮源条件下活性最高,脲酶在以尿素为氮源条件下活性表达最强;通过比较不同条件下硝酸盐还原酶活性及脲酶活性,发现脲酶活性远高于硝酸盐还原酶活性,这可能是以尿素为氮源条件下球形棕囊藻藻密度更高的主要原因。研究还发现,氮饥饿状态的球形棕囊藻对NH_4~+具有很高的初始吸收速率,8h左右将NH_4~+快速吸收耗尽,在随后的实验期间保持着较低且平稳的比生长速率,可见球形棕囊藻能快速吸收氨氮并储存在细胞内,当培养液中氮源耗尽后用于维持细胞的增长。
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| 关 键 词： | 球形棕囊藻 氮源 硝酸盐还原酶 脲酶 |
| 收稿时间： | 2018/1/5 0:00:00 |
| 修稿时间： | 2018/3/23 0:00:00 |
ABSORPTION AND UTILIZATION OF DIFFERENT NITROGEN SOURCES BY PHAEOCYSTIS GLOBOSA |
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| MENG Rui,SONG Xiu-Xian,LIU Shu-Y,JIANG Wen-Bin and YU Zhi-Ming.ABSORPTION AND UTILIZATION OF DIFFERENT NITROGEN SOURCES BY PHAEOCYSTIS GLOBOSA[J].Oceanologia Et Limnologia Sinica,2018,49(4):802-808. |
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| Authors: | MENG Rui SONG Xiu-Xian LIU Shu-Y JIANG Wen-Bin and YU Zhi-Ming |
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| Institution: | CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;University of Chinese Academy of Sciences, Beijing 100049, China,CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;University of Chinese Academy of Sciences, Beijing 100049, China,CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;University of Chinese Academy of Sciences, Beijing 100049, China,CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;University of Chinese Academy of Sciences, Beijing 100049, China and CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | |
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| Keywords: | Phaeocystis globosa nitrogen nitrate reductase urease |
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