Effects of grazing by Daphnia on algal carotenoids: Implications for paleolimnology

Reverse-phase thin layer chromatography was used to quantify algal carotenoid degradation resulting from grazing by Daphnia magna in suspensions of lyophilized Oscillatoria utermöhlii, O. limnetica, Anabaena flos-aquae and Synechococcus sp. Three samples were removed every 24 h from 0 h to 144 h and the carotenoid, carbon and nitrogen content of the remaining algal-fecal mixture was determined. Grazing by Daphnia reduced carotenoid concentrations by 30%–80% after 144 h, with myxoxanthophyll being the most labile pigment in experiments with filamentous cyanophytes. However, as a group, the carotenoids were more resistant to the combined effects of grazing by Daphnia and bacterial action than were particulate algal carbon (78% to 82% lost by 144 h) or nitrogen (86%–90%) during the period in which easily digestible material remained. The maximal extent of carotenoid enrichment relative to particulate carbon ranged from 150% to 300% of initial pigment concentrations but declined with repeated coprophagy. On the basis of these results and published pigment budgets, we conclude both that fecal transportation of pigments may be an important determinant of carotenoid accumulation rates and that carotenoid stratigraphies should record predator-mediated changes in zooplankton community structure, especially in oligotrophic conditions.