cDNA cloning and expression of a cytochrome P450 1A (CYP1A) gene from the hermaphroditic fish Rivulus marmoratus |
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Authors: | Lee Young-Mi Williams Tim D Jung Sang-Oun Lee Jae-Seong |
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Affiliation: | aDepartment of Environmental Science, Graduate School, Hanyang University, Seoul 133-791, South Korea bSchool of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom |
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Abstract: | We previously described the genomic structure of the cytochrome P450 1A (CYP1A) gene from the hermaphroditic fish Rivulus marmoratus [Kim, I.-C., Kim, Y.J., Yoon, Y.-D, Kawamura, S., Lee, Y.-S., Lee, J.-S., 2004a. Cloning of cytochrome P450 1A (CYP1A) genes from the hermaphroditic fish Rivulus marmoratus and the Japanese medaka Oryzias latipes. Mar. Environ. Res. 58, 125–129]. To further characterize R. marmoratus CYP1A, we cloned the cDNA sequence of a CYP1A gene from this species and also expressed its recombinant protein in an E. coli system. We exposed R. marmoratus to 4-nonylphenol, and found a small induction of CYP1A mRNA in the treated animals. In this paper, we discuss the characteristics of R. marmoratus CYP1A gene as well as its potential use in a biomonitoring assay. |
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Keywords: | Rivulus marmoratus CYP1A cDNA Expression Escherichia coli |
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