Nucleotide sequence of β-tubulin gene from the soft coralscleronephthya gracillimum (kükenthal) |
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Authors: | Seungshic Yum Seonock Woo Man Chang Taek-Kyun Lee Jun-Im Song |
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Institution: | (1) Southern Coastal Environment Research Division, South Sea Institute, KORDI, 656-830 Geoje, Korea;(2) Department of Biological Science, College of Natural Sciences, Ewha Womans University, 120-750 Seoul, Korea |
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Abstract: | We cloned the complete cDNA of the β-tubulin from the soft coral,Scleronephthya gracillimum (Kükenthal) (Alcyonacea, Octocorallia, Anthozoa, Cnidaria), via the random sequencing of a cDNA library and the 5′-rapid
amplification of cDNA end (RACE) technique. The full-length cDNA of theS. gracillimum β-tubulin comprised 1541 bp, not including the poly (A)+ stretch, also contained a complete open reading frame, which codes for a total of 445 amino acids. The amino acid residues
16-402 appeared to be in a state of conservation in a variety of animals. Northern blot analysis clearly demonstrated that
the sequence we have obtained is, indeed, the full-length cDNA of the β-tubulin gene inS. gracillimum. |
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Keywords: | Scleronephthya gracillimum soft coral β -tubulin cDNA library 5′ -RACE |
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