| Temperature Influences on the Expression of GFP Promoted by the Upstream Sequence of cpcB from Arthrospiraplatensis |
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| 作者姓名： | LU Yongzhong ZHANG Xuecheng |
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| 作者单位： | LU Yongzhong 1)，2)，and ZHANG Xuecheng1)，1) College of Marine Life Sciences，Ocean University of China，Qingdao 266003，P. R. China 2) Qingdao University of Science and Technology，Qingdao 266042，P. R. China |
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| 摘 要： | In order to investigate the regulation mechanism of the phycocyanin gene,a series of functional analyses of the upstream sequence of cpcB gene from Arthrospira platensis were conducted in E. coli with green fluorescent protein encoding gene(gfp) as the reporter. Results showed that the gfp gene could express at a high level under the promotion of the upstream sequence,suggesting the existence of some strong promoter elements in it. The expression of GFP was influenced by temperature. Higher temperature led to higher expression level. The bioinformatics analyses followed by mutation analyses on the secondary structure of translation ini-tiation region(TIR) revealed that RNA thermosensor might account for the temperature regulation.
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| 关 键 词： | 藻青菌 细胞计量术 藻青蛋白 基因突变 热敏元件 |
| 收稿时间： | 4 April 2006 |
| 修稿时间： | 2006-04-042007-01-03 |
Temperature influences on the expression of GFP promoted by the upstream sequence of <Emphasis Type="Italic">cpcB</Emphasis> from <Emphasis Type="Italic">Arthrospira platensis</Emphasis> |
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| LU Yongzhong ZHANG Xuecheng.Temperature Influences on the Expression of GFP Promoted by the Upstream Sequence of cpcB from Arthrospiraplatensis[J].Journal of Ocean University of China,2007,6(3):244-248. |
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| Authors: | Lu Yongzhong Zhang Xuecheng |
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| Institution: | 1. College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China;Qingdao University of Science and Technology, Qingdao 266042, P. R. China
2. College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China |
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| Abstract: | In order to investigate the regulation mechanism of the phycocyanin gene,a series of functional analyses of the upstream sequence of cpcB gene from Arthrospira platensis were conducted in E. coli with green fluorescent protein encoding gene(gfp) as the reporter. Results showed that the gfp gene could express at a high level under the promotion of the upstream sequence,suggesting the existence of some strong promoter elements in it. The expression of GFP was influenced by temperature. Higher temperature led to higher expression level. The bioinformatics analyses followed by mutation analyses on the secondary structure of translation ini-tiation region(TIR) revealed that RNA thermosensor might account for the temperature regulation. |
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| Keywords: | cyanobacterium flow cytometry mutation phycocyanin promoter RNA thermosensor |
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