| Histopathological Observation of Lymphocystis Disease and Lymphocystis Disease Virus （LCDV） Detection in Cultured Diseased Sebastes schlegeli |
| |
| 引用本文： | SHENG Xiuzhen ZHAN Wenbin XU Songjuan CHENG Shunfeng. Histopathological Observation of Lymphocystis Disease and Lymphocystis Disease Virus （LCDV） Detection in Cultured Diseased Sebastes schlegeli[J]. 中国海洋大学学报(英文版), 2007, 6(4): 378-382. DOI: 10.1007/s11802-007-0378-x |
| |
| 作者姓名： | SHENG Xiuzhen ZHAN Wenbin XU Songjuan CHENG Shunfeng |
| |
| 作者单位： | SHENG Xiuzhen，ZHAN Wenbin，XU Songjuan，and CHENG Shunfeng Laboratory of Pathology and Immunology of Aquatic Animals，LMMEC，Ocean University of China，Qingdao 266003，P. R. China |
| |
| 基金项目： | 国家自然科学基金;国家高技术研究发展计划(863计划) |
| |
| 摘 要： | Lymphocystis nodules occurring in the cultured sting fish Sebastes schlegeli were observed under light and electron microscope. Lymphocystis disease virus (LCDV) in the tissues of diseased fish was detected with indirect immunofluorescence test (IFAT). Results showed that lymphocystis cells had overly irregular nuclei, basophilic intracytoplasmic inclusion bodies with virions budding from the surface, and hyaline capsules outside the cell membrane. Numerous virus particles about 200 nm in diameter scat- tered in the cytoplasm, electron-dense particles 70-80 nm in diameter filled in perinuclear cisterna, and membrane-enveloped parti- cles with electron-dense core of 70-80 nm appeared around cellular nucleus. IFAT using monoclonal antibody against LCDV from Paralichthys olivaceus revealed that specific green fluorescence was present in the cytoplasm of lymphocystis cells, epithelium of stomach, gill lamellae, and muscular fibers under epidermis of S. schlegeli, just as that in the cytoplasm of lymphocystis cells of P. olivaceus, suggesting the presence of LCDV in these tissues.
|
| 关 键 词： | 组织病理学 超微结构 荧光免疫学 刺鱼 |
| 收稿时间： | 2007-03-21 |
| 修稿时间： | 2007-03-21 |
Histopathological observation of lymphocystis disease and lymphocystis disease virus (LCDV) detection in cultured diseased Sebastes schlegeli |
| |
| Sheng Xiuzhen,Zhan Wenbin,Xu Songjuan,Cheng Shunfeng. Histopathological observation of lymphocystis disease and lymphocystis disease virus (LCDV) detection in cultured diseased Sebastes schlegeli[J]. Journal of Ocean University of China, 2007, 6(4): 378-382. DOI: 10.1007/s11802-007-0378-x |
| |
| Authors: | Sheng Xiuzhen Zhan Wenbin Xu Songjuan Cheng Shunfeng |
| |
| Affiliation: | Laboratory of Pathology and Immunology of Aquatic Animals, LMMEC, Ocean University of China, Qingdao 266003, P. R. China |
| |
| Abstract: | Lymphocystis nodules occurring in the cultured sting fish Sebastes schlegeli were observed under light and electron microscope. Lymphocystis disease virus (LCDV) in the tissues of diseased fish was detected with indirect immunofluorescence test (IFAT). Results showed that lymphocystis cells had overly irregular nuclei, basophilic intracytoplasmic inclusion bodies with virions budding from the surface, and hyaline capsules outside the cell membrane. Numerous virus particles about 200 nm in diameter scat- tered in the cytoplasm, electron-dense particles 70-80 nm in diameter filled in perinuclear cisterna, and membrane-enveloped parti- cles with electron-dense core of 70-80 nm appeared around cellular nucleus. IFAT using monoclonal antibody against LCDV from Paralichthys olivaceus revealed that specific green fluorescence was present in the cytoplasm of lymphocystis cells, epithelium of stomach, gill lamellae, and muscular fibers under epidermis of S. schlegeli, just as that in the cytoplasm of lymphocystis cells of P. olivaceus, suggesting the presence of LCDV in these tissues. |
| |
| Keywords: | sting fish Sebastes schlegeli lymphocystis disease histopathology ultrastructure indirect immunofluorescence test |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
