褐篮子鱼消化道内分泌细胞的鉴别与定位

应用4种兔抗胃肠激素抗体和SABC免疫组织化学方法,对褐篮子鱼(Sigauns fuscesens)消化道中的内分泌细胞进行鉴别和定位。结果表明,Som细胞集中位于食道和胃部;PP细胞在幽门盲囊分布较多,少量分布于小肠和直肠;NPY细胞在幽门盲囊和小肠有少量分布;5-HT细胞在消化道各段均有分布,且数量较多。4种内分泌细胞的数量分布和形态特征存在一定的差异,对其可能的内分泌方式和生理作用进行了讨论。

Immunohistochemical studies on endocrine cells in the digestive tract of Sigauns fuscesens

By using immunohisochemistry method of Strept Avidin-Biotin-Complex,four kinds of antisera raised against rabbit hormones: somatostatin(Som),pancreatic polypeptide(PP),neuropeptide Y(NPY) and 5-hydroxytryptamine(5-HT) were used to indentify and locate the endocrine cells in the digestive tract of Sigauns fuscesens.The results showed that Som-immunoreactive cells were observed mainly in oesophagus and stomach;PP-immunoreactive cells were mainly located in the pyloricus sac,while sparsely in the small intestine and the rectum;NPY-immunoreactive cells were presented rarely in the pyloricus sac and the small intestine;5-HT-immunoreactive cells were distributed in all parts of the digestive tract with the higher density.The morphologic characteristics of the endocrine cells were described.The distribution feature of these cells and the relationship between their shape and the ways of endocrine were discussed.