| Evaluation of the damage in fish spermatozoa cryopreservation |
| |
| 作者姓名： | 李军 刘清华 张士璀 |
| |
| 作者单位： | [1]Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China [2]Department of Marine Biology, Ocean University of China, Qingdao 266003, China |
| |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| |
| 摘 要： |
|
| 关 键 词： | 冷冻损伤 低温贮藏 鱼肉 融化 电泳 |
| 收稿时间： | 2004-10-09 |
| 修稿时间： | 2005-07-10 |
Evaluation of the damage in fish spermatozoa cryopreservation |
| |
| Li Jun,Liu Qinghua,Zhang Shicui.Evaluation of the damage in fish spermatozoa cryopreservation[J].Chinese Journal of Oceanology and Limnology,2006,24(4):370-377. |
| |
| Authors: | Li Jun Liu Qinghua Zhang Shicui |
| |
| Institution: | (1) Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao, China;(2) Department of Marine Biology, Ocean University of China, 266003 Qingdao, China |
| |
| Abstract: | Cryodamages occur during sperm cryopreservation. Cryopreservation of fish sperm usually results in marked decrease in sperm quality, such as swelling or disruption of the plasma membrane, mitochondrial dysfunction, diminished sperm motility, impaired velocity, shorter motility period, denaturation, and release of some enzymes from spermatozoa. In this paper, damages in morphology, physiology, biochemistry and metabolism, and genetic integrity of fish semen after cryopreservation are discussed. New approaches in assessment of fish thawed sperm quality such as computer assisted sperm analysis, flow cytometic analysis combined with fluorescent probes and single cell gel electrophoresis are also briefly reviewed. |
| |
| Keywords: | cryodamage cryopreservation fish sperm freezing and thawing |
| 本文献已被 维普 万方数据 SpringerLink 等数据库收录！ |
|
