B[a]P–DNA binding in early life-stages of Atlantic tomcod: population differences and chromium modulation |
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Authors: | C. Sorrentino N. K. Roy R. C. Chambers S. C. Courtenay I. Wirgin |
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Affiliation: | a Nelson Institute of Environmental Medicine, School of Medicine, New York University, 57 Old Forge Road, Tuxedo, NY 10987, USA;b Howard Marine Sciences Laboratory, National Marine Fisheries Service, 74 Magruder Road, Highlands, NJ 07732, USA;c Fisheries & Oceans Canada, Gulf Fisheries Centre, P.O. Box 5030, Moncton, NB, Canada E1C 9B6 |
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Abstract: | Atlantic tomcod (Microgadus tomcod) from the Hudson River (HR) are resistant at the molecular and organismic levels to the effects of exposure to dioxin-like aromatic hydrocarbon (AH) compounds, but much less so to benzo[a]pyrene (B[a]P). The aims of this study were to determine in early life-stages of tomcod exposed to B[a]P: (1) if DNA binding levels differed between fish from the HR and Miramichi River (MR), and (2) if co-exposure to chromium could modulate this genotoxic effect. After exposure to [3H]B[a]P alone, DNA-bound radioactivity was 5–10-fold higher in embryos and larvae of MR than HR descent. Co-exposure to chromium modulated DNA binding levels in offspring of both populations. In MR embryos, co-exposure to chromium inhibited B[a]P uptake. These results demonstrated resistance to the genotoxic effects of B[a]P in early life stages of HR tomcod at an ecologically important endpoint and suggest the ability of chromium to modulate AH-induced genotoxicity. |
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Keywords: | Resistance Genotoxicity Early life-stages Hydrocarbons Metal– organic interactions DNA-binding |
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