| 印度尼西亚峇淡岛海域鱾新本尼登虫（Neobenedenia girellae）形态学及28S rRNA分子鉴定 |
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| 引用本文： | 乔莹,马笑晚,邵彦翔,陈超. 印度尼西亚峇淡岛海域鱾新本尼登虫（Neobenedenia girellae）形态学及28S rRNA分子鉴定[J]. 海洋学报, 2020, 42(6): 119-126. DOI: 10.3969/j.issn.0253-4193.2020.06.014 |
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| 作者姓名： | 乔莹 马笑晚 邵彦翔 陈超 |
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| 作者单位： | 1.自然资源部第四海洋研究所，广西 北海 536000 |
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| 基金项目： | 广西壮族自治区青年自然科学基金（2018GXNSFBA050032）；广西壮族自治区科技基地和人才专项（2018AD19365）；自然资源部第四海洋研究所博士启动基金（201803）；大黄鱼育种国家重点实验室开放课题（LYC2019RS01）。 |
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| 摘 要： | 本研究通过结合形态学与DNA分子标记等生物学手段,对印度尼西亚峇淡岛海域海水鱼类养殖中的本尼登虫(Isolate Batam, IB)进行形态学分析和种类鉴定。IB虫株在形态上与鱾新本尼登虫(Neobenedenia girellae)相似,属于新本尼登属。28S rRNA序列扩增得到序列为394 bp,与其他本尼登虫属比对相似性在85.86%～99.47%之间。进化树构建结果显示IB虫株与其他珻氏新本尼登虫(Neobenedenia melleni)和鱾新本尼登虫构成一簇,而本尼登虫自成一簇。综上所述,本研究结果支持鱾新本尼登虫与珻氏新本尼登虫为同种异名的分类学观点,并将IB虫株定种为鱾新本尼登虫。
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| 关 键 词： | 新本尼登虫 形态鉴定 28S rRNA 印度尼西亚 |
| 收稿时间： | 2019-11-14 |
| 修稿时间： | 2020-02-22 |
Morphology and 28S rRNA identification of Neobenedenia girellae in cage aquaculture at the Batam Island, Indonesia |
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| Qiao Ying,Ma Xiaowan,Shao Yanxiang,Chen Chao. Morphology and 28S rRNA identification of Neobenedenia girellae in cage aquaculture at the Batam Island, Indonesia[J]. Acta Oceanologica Sinica (in Chinese), 2020, 42(6): 119-126. DOI: 10.3969/j.issn.0253-4193.2020.06.014 |
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| Authors: | Qiao Ying Ma Xiaowan Shao Yanxiang Chen Chao |
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| Affiliation: | 1.Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai 536000, China2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China |
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| Abstract: | In present study, the morphology and species identification of the Neobenedenia parasites (Isolate Batam/IB) in the cage aquaculture were studied via morphological and molecular marker methods at Batam Island, Indonesia. The isolate Batam is morphological similar with Neobenedenia girellae, and it is considered to belong to the Neobenedenia genera. 394 bp of the 28S rRNA was amplified, and its similarity with other genera was between 85.86%-99.47%. The phylogenetic tree shows that IB, Neobenedenia melleni and Neobenedenia girellae are in a same cluster, and other Benedeniinae species form their own cluster. To sum up, IB is identified as the specie of Neobenedenia girellae and we support the theory that N. melleni and N. girellae are synonyms. |
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| Keywords: | Neobenedenia morphological identification 28S rRNA Indonesia |
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