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STUDY ON MIXIS POTENTIAL OF ROTIFER RESTING EGGS ( BRACHIONUS PLICATILIS ) WITH DIFFERENT COLLECTION TIMES AND DIFFERENT PRESERVATION PERIODS
作者姓名:周利  郑严  相建海
作者单位:ZHOU Li ,,ZHENG Yan ,XIANG Jian Hai  (Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China) (Institute of Molecular Agrobiology,an Affiliate of the National University of Singapore,117604 Singapore)
基金项目:ProjectwassupportedbyagrantfromtheSingapore ChinaBiotechnologyProgramoftheInstituteofMolecularAgrobiology anaffiliateoftheNati
摘    要:INTRODUCTIONRestingeggs,theproductofsexual(mictic)reproductionofmonogonontrotiferslikeBrachio nusplicatilisorB .rotundiformis,canbereadilystoredandusedwhenneededtostartmassculturesfortheproductionoflivefoodsforlarvicultureofmarinefishesandcrustaceans.The…


STUDY ON MIXIS POTENTIAL OF ROTIFER RESTING EGGS ( BRACHIONUS PLICATILIS ) WITH DIFFERENT COLLECTION TIMES AND DIFFERENT PRESERVATION PERIODS
Abstract:The present study investigated the possible changes in the mixis potential of rotifer resting eggs produced by a single stock of Brachionus plicatilis and collected and preserved annually from 1985-1998. Several clones derived from each batch of resting eggs were cultured under the same conditions for 21 days. The percentage of clones appearing resting eggs and the average yield of resting eggs produced from each clone were recorded and statistically analyzed to find the differences between the mixis potential of those resting egg batches. Results showed that different batches of resting eggs had different mictic levels among their descendent clones; but no regular relationship was found between the mixis potential of resting eggs and their collection times/preservation periods. Several internal and external factors that might affect the mixis potential of resting eggs were discussed.
Keywords:Brachionus plicatilis    resting eggs  mixis potential  collection times  preservation periods
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