Variation in Rubisco and other photosynthetic parameters in the life cycle of Haematococcus pluvialis |
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Authors: | Zhangfan Chen Guangce Wang Jianfeng Niu |
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Institution: | 1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China 2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China
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Abstract: | Cells of Haematococcus pluvialis Flot. et Will were collected in four different growth phases. We quantified the initial and total enzyme activity of ribulose-1,5-bisphosphate
carboxylase (Rubisco) in crude extracts, and the relative expression of large-subunit ribulose-1,5-bisphosphate caboxylase
/ oxygenase (rbcL) mRNA. We measured the ratio of photosynthetic rate to respiration rate (P/R), maximal effective quantum yield of photosystem
II (F
v/F
m), electron transport rate (ETR), actual photochemical efficiency of PSII in the light (PSII), and non-photochemical quenching
(NPQ). Green vegetative cells were found to be in the most active state, with a relatively higher P/R ratio. These cells also
displayed the lowest NPQ and the highest F
v/F
m, ETR, and PSII, indicating the most effective PSII. However, both Rubisco activity and rbcL mRNA expression were the lowest measured. In orange resting cysts with relatively lower P/R and NPQ, Rubisco activity and
rbcL expression reached a peak, while F
v/F
m, ETR, and ΦPSII were the lowest measured. Taking into account the methods of astaxanthin induction used in industry, we suggest
that Rubisco may participate in astaxanthin accumulation in H. pluvialis. A continuous and sufficient supply of a carbon source such as CO2 may therefore aid the large scale production of astaxanthin. |
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Keywords: | Haematococcus pluvialis Rubisco astaxanthin life cycle growth phase |
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