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Cloning and sequence analysis of the self-fertilizing fish Rivulus marmoratus immediate early gene c-fos
Authors:Yan Li  Il-Chan Kim  Young Ja Kim  Moon Kyoo Kim  Yong-Dal Yoon  Yong-Sung Lee  Jae-Seong Lee  
Institution:a Department of Biochemistry, College of Medicine, Hanyang University, Seoul 133-791, Republic of Korea;b Department of Environmental Science, Graduate School, Hanyang University, Seoul 133-791, Republic of Korea;c Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 133-791, Republic of Korea
Abstract:We have cloned the proto-oncogene c-fos from a self-fertilizing fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae) after screening of R. marmoratus λGEM-11 genomic DNA library, and sequenced over 12 kb including all exons, introns and the promoter region. The R. marmoratus c-fos gene consisted of one noncoding exon and four exons with high similarity to those of fugu and mammals. We sequenced ≈7 kb of the R. marmoratus c-fos gene promoter region to gain a better understanding of the molecular anatomy of the immediate response of this gene upon cellular damage. In the promoter region, R. marmoratus c-fos gene has seven xenobiotic response elements (XREs) and eight metal response elements (MREs) as well as two estradiol (E2), 4 NFκB, 2 CarG, 2 prolactin (PRL) motifs and one pit1 site, while the 3-UTR of this gene contains the estrogen response element (ERE). The seven XRE and eight MRE motifs raise the possibility of its regulation by exposure to environmental pollutants. In this paper, we discuss the gene structure of R. marmoratus c-fos gene and compare its promoter region with those of other organisms' c-fos genes. We propose its potential use in ecotoxicology.
Keywords:Rivulus marmoratus  c-fos  Gene  Cloning  Promoter  MRE  XRE
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