CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.     

Discriminating between pore‐filling load and bed‐structure load: a new porosity‐based method,exemplified for the river Rhine

Sediments contained in the river bed do not necessarily contribute to morphological change. The finest part of the sediment mixture often fills the pores between the larger grains and can be removed without causing a drop in bed level. The discrimination between pore‐filling load and bed‐structure load, therefore, is of practical importance for morphological predictions. In this study, a new method is proposed to estimate the cut‐off grain size that forms the boundary between pore‐filling load and bed‐structure load. The method evaluates the pore structure of the river bed geometrically. Only detailed grain‐size distributions of the river bed are required as input to the method. A preliminary validation shows that the calculated porosity and cut‐off size values agree well with experimental data. Application of the new cut‐off size method to the river Rhine demonstrates that the estimated cut‐off size decreases in a downstream direction from about 2 to 0·05 mm, covariant with the downstream fining of bed sediments. Grain size fractions that are pore‐filling load in the upstream part of the river thus gradually become bed‐structure load in the downstream part. The estimated (mass) percentage of pore‐filling load in the river bed ranges from 0% in areas with a unimodal river bed, to about 22% in reaches with a bimodal sand‐gravel bed. The estimated bed porosity varies between 0·15 and 0·35, which is considerably less than the often‐used standard value of 0·40. The predicted cut‐off size between pore‐filling load and bed‐structure load (D_c,p) is fundamentally different from the cut‐off size between wash‐load and bed‐material load (D_c,w), irrespective of the method used to determine D_c,p or D_c,w. D_c,w values are in the order of 10^?1 mm and mainly dependent on the flow characteristics, whereas D_c,p values are generally much larger (about 10⁰ mm in gravel‐bed rivers) and dependent on the bed composition. Knowledge of D_c,w is important for the prediction of the total sediment transport in a river (including suspended fines that do not interact with the bed), whereas knowledge of D_c,p helps to improve morphological predictions, especially if spatial variations in D_c,p are taken into account. An alternative to using a spatially variable value of D_c,p in morphological models is to use a spatially variable bed porosity, which can also be predicted with the new method. In addition to the morphological benefits, the new method also has sedimentological applications. The possibility to determine quickly whether a sediment mixture is clast‐supported or matrix‐supported may help to better understand downstream fining trends, sediment entrainment thresholds and variations in hydraulic conductivity.     

U–Pb SHRIMP zircon geochronology and T–t–d history of the Kampa Dome, southern Tibet

Structural, petrographic and geochronologic studies of the Kampa Dome provide insights into the tectonothermal evolution of orogenic crust exposed in the North Himalayan gneiss domes of southern Tibet. U–Pb ion microprobe dating of zircons from granite gneiss exposed at the deepest levels within the dome yields concordia ²⁰⁶Pb/²³⁸U age populations of 506 ± 3 Ma and 527 ± 6 Ma, with no evidence of new zircon growth during Himalayan orogenesis. However, the granite contains penetrative deformation fabrics that are also preserved in the overlying Paleozoic strata, implying that the Kampa granite is a Cambrian pluton that was strongly deformed and metamorphosed during Himalayan orogenesis. Zircons from deformed leucogranite sills that cross-cut Paleozoic metasedimentary rocks yield concordant Cambrian ages from oscillatory zoned cores and discordant ages ranging from ca. 491–32 Ma in metamict grains. Since these leucogranites clearly post-date the metasedimentary rocks they intrude, the zircons are interpreted as xenocrysts that are probably derived from the Kampa granite. The Kampa Dome formed via a series of progressive orogenic events including regional ~ N–S contraction and related crustal thickening (D₁), predominately top-to-N ductile shearing and crustal extension (D₂), top-to-N brittle–ductile faulting and related folding on the north limb of the dome, localized top-to-S faulting on the southern limb of the dome, and crustal doming (D₃), and continued N–S contraction, E–W extension and doming (D₄). Structural and geochronologic variability amongst adjacent North Himalayan gneiss domes may reflect changes in the magnitude of crustal exhumation along the North Himalayan antiform, possibly relating to differences in the mid-crustal geometry of the exhuming fault systems.     

台湾海峡纽形动物初报

首次报道台湾海峡产的纽形动物，包括沙栖原细首纽虫、浮游拟脑纽虫、椒状岩田纽虫、中华平裂纽虫和马顿氏潘丁纽虫，共５种。除椒状岩田纽虫在琉球群岛有报道外，均为东海新记录。     

Images of the seabed in the Faroe-Shetland Channel from commercial 3D seismic data

A large number of 3D deep seismic surveys in the Faroe-Shetland Channel gives continuous coverage over most of the region. These surveys were designed primarily to image depths in excess of 4 km, use low frequency sources and are recorded at low temporal sample rates. However, commercial 3D data can generate highly detailed images of the seabed due to the high spatial sample rate, typically 12.5 m. This is particularly true in waters below 200 m. Despite geophysical artefacts, the images reveal that there are a number of sedimentary processes at work adjacent to and within this channel. On the West Shetland Shelf, iceberg scouring and moraines reflect the impact of glaciation. On the West Shetland slope there is clear evidence for down-slope processes, such as debris flows, linear erosion channels, basal fans and (one case) slope failure. Along-slope processes are also active as indicated by the presence of sediment waves and contourite mounds. On the floor of the basin, polygonal cracking can be observed. The most spectacular feature appears to be the Judd Deeps, a system of cliffs approximately 200 m high and 40 km across. Traditionally, seabed investigation has been performed using high-resolution surveys. This study shows that deep exploration data can also provide useful images of the seafloor.     

扇贝外套腔内的共栖生纤毛虫 Ⅰ.蠕状康纤虫(Cohnilembus verminus)的形态学研究

研究扇贝外套腔内栖生的纤毛虫一蠕状康纤虫Cohnilembusverminus（Muller，1786）的形态、核器及纤毛图式。结果显示其口器结构为；M1呈单列结构，约占体长的1／2，M2由4组毛基粒构成，PM起自M2末端，Sc约由5个毛基粒构成。此外，还列表比较了不同作者报导的C．verminus种群及其它6种已知的康纤虫的主要性状特征。     

Seabed Morphology and Pollution Along the Bagnoli Coast (Naples, Italy): a Hypothesis for Environmental Restoration

Abstract. The interaction of coastal and submarine morphology with the hydrodynamic regimes exerts a control on coastal dynamic processes, conditioning the dispersion of sediments and potential pollutants existing in the area. Thus, the study of such parameters is useful in order to define environmental threats. Within the submerged sector of the Bagnoli coast and particularly in the southern part of the investigated area, there are sediment groups composed of very fine sands located in low-energy zones due to wave refraction and diffraction; they can also be found on the sea bottom and on the man-made structures typical of this zone. These areas show high pollutant levels of N, P, Cu, Fe, Mn, Ni, Pb, Zn, Cd, polyaromatic hydrocarbons (PAHs) and polychlorinated bi-phenyls (PCBs). The northern area, a place of high-energy hydrodynamic processes, also shows high concentration levels of pollutants due to the presence of secondary cell circulation.
Morphological research and analysis of textural characteristics of bottom sediments along the Bagnoli coast allowed the actual processes and their evolution in space and time to be defined. It has also been possible to correlate such processes to the seabed morphology system, the wave formations which affect the coast, the complex system of sediment transport, as well as to the man-made interventions in the area. The results of recent chemical analyses of beach sediments and bottom sediments off the Bagnoli coast were also incorporated. They prove the presence of heavy metals, PAHs and PCBs in high and sometimes very high concentrations. Finally, based on the results of research and analyses, a hypothesis for an intervention for environmental restoration has been formulated in order to renaturalise the coast through dredging and treatment of the sand, both on the seabed and on the emerged beach.     

日照近海金乌贼生物学的初步研究

为给金乌贼渔业的可持续发展提供依据,通过形态学观察及生物学测定,初步研究了山东日照岚山头近海金乌贼Sepia esculentaHoyle,1885的外部形态特征和生物学特性(胴长与体重组成、生长参数相关关系等),并结合相关资料,对其生活史进行了讨论。研究结果表明:金乌贼胴长(ML)与体重(TW)之间的关系为:TW幼体=6.0×10-4ML2.762 6,TW♀=6.0×10-4ML2.767 1,TW♂=12.0×10-4ML2.585 6;金乌贼为异速生长型,其生长迅速、个体生长差异较大;5~6月为金乌贼繁殖季节,成体交配产卵后死亡,属1年生头足类。     

Comparison between zooplankton data collected by the Continuous Plankton Recorder survey in the English Channel and by WP-2 nets at station L4, Plymouth (UK)

Zooplankton sampling has been carried out by the Continuous Plankton Recorder (CPR) survey since the 1930s enabling the study of long-term changes in plankton populations, the elucidation of seasonal patterns of abundance, and more recently providing zooplankton biomass estimates for ecosystem models. Data for zooplankton abundance collected by CPR tows in the Western English Channel (between 1988 and 1998) were compared to vertically integrated samples collected from station L4 off Plymouth, UK. Comparisons were made for locally abundant copepods (including Acartia, Calanus, Para/Pseudocalanus, Centropages, Oithona and Temora) collected by CPR and WP-2 nets. All dominant species recorded at L4 were also common to the CPR data. However, the position of the taxa in the two datasets was not equivalent. Seasonal cycles revealed by CPR data were significantly similar to those recorded throughout the water column at L4 for most taxa. However, absolute levels of abundance differed for the two datasets: abundances were underestimated by CPR samples when compared to those of vertically integrated samples by a factor of between 2 and 35, with the exception of Centropages. The differing mesh sizes (200 and 270 μm) of the WP-2 net and CPR mesh could only partially explain these differences in abundance, implying that the behaviour of individual taxa and their depth in the water column also influenced the abundance recorded.