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文昌鱼鳃组织原代培养 总被引:1,自引:0,他引:1
采用组织块培养法培养文昌鱼(Branchiostoma belcheri japonicum)鳃细胞,使用L15,M199,Eargle'sMEM为基础培养基,加入一定量的NaCl及5%,10%,20%等不同体积分数的牛血清.经条件优化后得到最适合的培养条件为2L15+20%FBS+0.45%NaCl,在26~28℃条件下培养,组织块贴壁后12 h内就有至少4类上皮样细胞从中迁移出来,这些细胞在前3天迅速增殖,到第6天基本上达到平稳期,往后细胞开始凋亡,最长可以存活近两个星期.鳃细胞的原代培养为文昌鱼细胞体外培养的深入研究提供了实验依据. 相似文献
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四川大沟流域土壤活性铝含量及其对连香树群落的影响 总被引:7,自引:2,他引:7
研究了四川茂县大沟流域不同高度,不同土壤类型的土壤活性铝含量和土壤活性铝含量变化与连香树群落生长的关系,并对土壤活性铝变化原因及产生铝毒的有关问题进行了讨论。 相似文献
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Barbara Calcinai Carlo Cerrano Nozomu Iwasaki & Giorgio Bavestrello 《Marine Ecology》2008,29(2):273-279
Precious corals represent peculiar substrata for several species of boring sponges that exploit their carbonatic scleraxis, strongly decreasing their commercial value. Here we describe a new species of the genus Alectona from Japan recorded in a colony of Paracorallium japonicum (Kishinouye, 1903). The spicular complement of the new species consists of diactinal spicules covered by mushroom-like tubercles, often modified into styloid forms, and fusiform amphiasters with two or sometimes four verticils, each generally made up of six short tubercled rays. A complete survey of the literature on boring sponges recorded in precious corals in the Mediterranean Sea and Pacific Ocean indicates that the species of the family Alectonidae are the most strictly associated to this kind of substratum. Their world distribution, in fact, partially or totally overlap that of their coral hosts. 相似文献
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