日本鬼鲉蜇伤与P物质和生长抑素的关系及其粗毒对免疫细胞活性影响

１９９７年６月在山东省日照市岚山头附近海域收集日本鬼ｙｏｕ,采用放射凤学方法检测了注射毒腺粗提物大鼠局部肌肉组织、血浆、疹髓、延髓、中脑、下丘脑及免疫器官内Ｐ物质和生长抑素含量变化;用ＭＴＴ法检测了朱粗提物在整体和离体条件下对免疫细胞活性的影响;用生化方法检测了毒腺组织中透明质酸酶活性。结果表明,与对照组大鼠相比,实验组大鼠局部肌肉及延髓、中脑、胸腺中脾脏中Ｐ物质含量增加,而下丘脑中Ｐ物质含量下降     

日本鬼魀蜇伤与P物质和生长抑素的关系及其粗毒对免疫细胞活性影响

1997年 6月在山东省日照市岚山头附近海域收集日本鬼 ,采用放射免疫学方法检测了注射毒腺粗提物大鼠局部肌肉组织、血浆、脊髓、延髓、中脑、下丘脑及免疫器官内P物质和生长抑素含量变化 ;用MTT法检测了毒腺粗提物在整体和离体条件下对免疫细胞活性的影响 ;用生化方法检测了毒腺组织中透明质酸酶活性。结果表明 ,与对照组大鼠相比 ,实验组大鼠局部肌肉及延髓、中脑、胸腺和脾脏中P物质含量增加 ,而下丘脑中P物质含量下降。生长抑素在中脑、延髓、脊髓、胸腺中显著增高。毒腺粗提物对胸腺细胞和脾细胞的活性具有显著的抑制作用。提示日本鬼蜇伤造成局部症状及其伴随的恶心、呕吐、心慌、胸闷等全身症状和继发感染可能与P物质和生长抑素含量变化有关。     

Comparative analysis of methods for concentrating venom from jellyfish Rhopilema esculentum Kishinouye

In this study, several methods were compared for the efficiency to concentrate venom from the tentacles of jellyfish Rhopilema esculentum Kishinouye. The results show that the methods using either freezing-dry or gel absorption to remove water to concentrate venom are not applicable due to the low concentration of the compounds dissolved. Although the recovery efficiency and the total venom obtained using the dialysis dehydration method are high, some proteins can be lost during the concentrating process. Comparing to the lyophilization method, ultrafiltration is a simple way to concentrate the compounds at high percentage but the hemolytic activities of the proteins obtained by ultrafiltration appear to be lower. Our results suggest that overall lyophilization is the best and recommended method to concentrate venom from the tentacles of jellyfish. It shows not only the high recovery efficiency for the venoms but high hemolytic activities as well.     

Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye

The present work is the first report of the biochemical characterization of the venom from nematocysts of the jellyfish Rhopilema esculentum Kishinouye. The nematocysts were isolated by autolysis and centrifugation and separated by flow cytometry. Four types of nematocysts were identified: mastigophores, euryteles, and atrichous and holotrichous isorhiza. SDS-PAGE and amino acid analyses demonstrated that most of the proteins in the nematocyst extract were between 10 kDa and 40 kDa, and that glutamic acid was the main amino acid. A hemolytic activity assay showed that the activity of the nematocyst venom (RNV) was strongest in Tris-HCl buffer (50 mmol/L, pH 7.8, 5% glycerol, 0.5 mmol/L EDTA, 0.1 mol/L NaCl). The hemolytic activity was related to protein concentration and the HU50 against chicken erythrocytes was 0.91 μg/mL.     

Comparative analysis of methods for concentrating venom from jellyfish Rhopilema esculentum Kishinouye

In this study, several methods were compared for the efficiency to concentrate venom from the tentacles of jellyfish Rhopilema esculentum Kishinouye. The results show that the methods using either freezing-dry or gel absorption to remove water to concentrate venom are not applicable due to the low concentration of the compounds dissolved. Although the recovery efficiency and the total venom obtained using the dialysis dehydration method are high, some proteins can be lost during the concentrating process. Comparing to the lyophilization method, ultrafiltration is a simple way to concentrate the compounds at high percentage but the hemolytic activities of the proteins obtained by ultrafiltration appear to be lower. Our results suggest that overall lyophilization is the best and recommended method to concentrate venom from the tentacles of jellyfish. It shows not only the high recovery efficiency for the venoms but high hemolytic activities as well. Supported by the Award Foundation of Scientific Research for Excellent Young and Middle-age Scientist of Shandong Province (No. 2006BS07003) and the Knowledge Innovation Project of Chinese Academy of Sciences (KZCX2-YW-R-104)