Fully coupled elastoplastic hydro-mechanical analysis of unsaturated porous media using a meshfree method

This paper presents the first application of an advanced meshfree method, ie, the edge-based smoothed point interpolation method (ESPIM), in simulation of the coupled hydro-mechanical behaviour of unsaturated porous media. In the proposed technique, the problem domain is spatially discretised using a triangular background mesh, and the polynomial point interpolation method combined with a simple node selection scheme is adopted for creating nodal shape functions. Smoothing domains are formed on top of the background mesh, and a constant smoothed strain, created by applying the smoothing operation over the smoothing domains, is assigned to each smoothing domain. The deformation and flow models are developed based on the equilibrium equation of the mixture, and linear momentum and mass balance equations of the fluid phases, respectively. The effective stress approach is followed to account for the coupling between the flow and deformation models. Further coupling among the phases is captured through a hysteretic soil water retention model that evolves with changes in void ratio. An advanced elastoplastic constitutive model within the context of the bounding surface plasticity theory is employed for predicting the nonlinear behaviour of soil skeleton. Time discretisation is performed by adopting a three-point discretisation method with growing time steps to avoid temporal instabilities. A modified Newton-Raphson framework is designed for dealing with nonlinearities of the discretised system of equations. The performance of the numerical model is examined through a number of numerical examples. The state-of-the-art computational scheme developed is useful for simulation of geotechnical engineering problems involving unsaturated soils.     

Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.     

试论新世纪地理信息科学的发展

本文重点分析了当前国内外地理信息科学的形成与发展趋势和地理信息科学不断发展的历程 ,提出近期发展的前沿 ,以及当前地理信息产业化面临的一些重要问题。最后提出对地理信息科学发展的意见。     

Canonical theory of perturbations using eccentric anomaly as independent variable

It is shown in this paper how to build a canonical transformation of variables, so that the eccentric anomaly becomes the new independent variable. In the case of eccentric elliptical orbits it changes the equations of motion so, that they can be integrated analytically to any order of approximation comparatively easy.