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排序方式: 共有113条查询结果,搜索用时 15 毫秒
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日本沼虾黑鳃病几种同工酶的变化与病理分析 总被引:10,自引:0,他引:10
酯酶(EST),过氧化物酶(POD),乳酸脱氢酶(LDH)和苹果酸脱氢酶(MDH)同工酶在日本沼虾中存在着组织和器官的特异性,患黑鳃病后酶带发生显著变化。在肌肉、肝脏、心脏和鳃中病虾较健康虾的LDH酶谱分别缺失了2、6、2和1条酶带,同时肝脏中新增了1条酶带;POD酶谱分别缺失了0、7、4和2酶带,肌肉中新增了2条酶带;EST酶谱分别缺失了0、2、3和1条酶带,肌肉、心脏和鳃中分别新增了1、2和2条酶带;MDH酶谱分别缺失了0、2、1和1条酶带,肌肉和脏脏中各新增了1条酶带。 相似文献
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随着DNA序列测定技术的飞速发展,人们可以在很短的时间内获得某种生物基因组的全序列。生物体的生命活动主要依赖于基因组的产物——蛋白质组,但是蛋白质组的研究相对于基因组来说却要复杂得多。蛋白质组技术是在细胞内整体蛋白质水平上进行研究,从蛋白质整体活动的角度来研究生命活动的规律。综述了蛋白质组和蛋白质组学出现的背景、起源、概念、研究技术、研究内容、面对的挑战、开展该项研究的意义、陆生动植物和微生物蛋白质组学的研究进展及海洋生物蛋白质组学的研究概况。目前,普通生物方面的蛋白质组工作开展得较多,海洋生物方面却相对滞后。种种迹象表明,开展海洋生物蛋白质组学研究,对揭示生命的奥秘、促进海洋资源的开发都有重大的推动作用。 相似文献
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Margaret C. Graham John G. Farmer Ian W. Oliver Angus B. MacKenzie Robert M. Ellam 《中国地球化学学报》2006,25(B08):112-112
Natural uranium has three isotopes, ^238U, ^235U and ^234U, with natural abundances of 99.27 atom %, 0.72% and 0.0055%, respectively. Only ^235U is fissile and the production of nuclear fuel and nuclear weapons involves enrichment of uranium in ^235U. This process also results in separation of ^234U from ^238U, leaving depleted uranium (DU), with typical ^234U/^238U and ^235U/^238U activity ratios of about 0.19 and 0.013, respectively, as a waste product. The high density, high melting and boiling points and chemical stability of uranium and the availability of DU in relatively pure form mean that DU has many uses, including armour-piercing munitions. Such munitions have been developed in the UK since the 1960s and testing has been carried out by the Ministry of Defence (MoD) at firing ranges such as Dundrennan, SW Scotland and Eskmeals, NW England. The firing of DU munitions can result in the dispersion of DU and its combustion products (oxides) as aerosols or as larger fragments, with the potential for human exposure either directly at the site of detonation or via post-depositional migration in the environment. The aim of this work was to investigate the potential environmental mobility of DU by characterizing the associations of U in soil porewaters with increasing distance from a firing site. To this end, several soil cores located down-wind of the firing site at Dundrennan, near Kirkcudbright, SW Scotland, were collected in May 2006. These were sectioned on-site into 1- or 2-cm depth intervals and porewaters were isolated by centfifugation (10 minutes; 8873 g) on return to the laboratory. Following filtration through 0.2-micron cellulose nitrate filters, the porewaters were analyzed by ICP-QMS (U concentration) and ICP-OES (Fe, Al, Ca, Mg, Mn concentrations). Sub-samples were also subjected to centrifugal ultrafiltration (100, 30, and 3 kD) and to gel electrophoretic fractionation (agarose; 0.045 M Tris-borate; 20 mA, 30 minutes). Results showed that U was present at up to 4 μg/L in the soil porewater and that the associations of U varied with sample location and soil depth. 相似文献
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GAO Hongwei) )* MAO Mao) LIANG Chengzhu) LIN Chao) XIANG Jianhai)) Institute of Oceanology Chinese Academy of Sciences Qingdao P. R. China ) College of Food Science Engineering Ocean University of China P. R. China ) Sh ong Entry-Exit Inspection Quarantine Bureau of P. R. China P. R. China 《中国海洋大学学报(英文版)》2009,8(1):95-99
Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied in the detection of the end point temperature (EPT) of thermal denatured protein in fish and meat in this study. It was also used in studying the thermal denatured temperature range of proteins in salmon and chicken meat. The results show that the temperature ranges of denatured proteins were from 65 ℃ to 75 ℃ , and these temperature ranges were influenced by the processing methods. Through SDS-PAGE, the features of repeated he... 相似文献
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6种海洋致病性弧菌36kDa外膜蛋白特性分析 总被引:3,自引:0,他引:3
研究本着筛选和制备弧菌共同外膜蛋白亚单位疫苗,用十二烷基肌氨酸钠抽提并结合超速离心的方法提取了鳗弧菌(Vibrio anguillarum)、鱼肠道弧菌(V.ichthyoenteri)、溶藻弧菌(V.alginolyticus)、副溶血弧菌(V.parahaemolyticus)、哈维氏弧菌(V.harveyi)和创伤弧菌(V.vulnificus)6种海洋致病性弧菌的主要外膜蛋白, SDS-PAGE图谱比较发现这6种弧菌都存在36 kDa的外膜蛋白条带.通过电泳洗脱分离纯化这6种弧菌的36 kDa外膜蛋白,并制备了鳗弧菌菌株SJ060621的36 kDa外膜蛋白多抗.ELISA和Western-blot印迹结果显示,仅鳗弧菌SJ060621、S010610-1和哈维氏弧菌的36 kDa外膜蛋白存在免疫交叉反应;双向电泳分析了鳗弧菌SJ060621、S010610-1、哈维氏弧菌和溶藻弧菌的36 kDa外膜蛋白,2-DE图谱显示出鳗弧菌SJ060621和S010610-1均由3种等电点相近的蛋白组成,存在很高的同源性,2株鳗弧菌与溶藻弧菌和哈维氏弧菌在蛋白质组成数量和等电点上都存在较大的差异,结果说明通过主要外膜蛋白的分子量和免疫特性研究,可为筛选不同弧菌共同外膜蛋白的亚单位疫苗提供有利参考. 相似文献