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When cultured under certain environmental conditions (25°C, light intensity 80 μmol/m2·s, LD 12/12, in enriched seawater medium with 7×10−4 mol/L NO3−N, 1.56×10−4 mol/L, PO4−P and supplements of other elements like Mn, Fe, I, etc.), male and female gametophytes ofU. pinnatifida kept growing vegetatively and propagated fast at average daily fresh weight increase rate of about 20%. The empirical formulaG
m=G
o·3m was established to estimate the output of vegetative gametophytes. Vigorous vegetative gametophyte cells began to form reproductive
structures (oogonium and spermatangium, when the temperature was lower than 25°C and other environmental factors were kept
optimal. The sufficient supply of gametophyte cells provided enough seeds for raisingUndaria sporelings on production scale. Controlled cross-breeding experiments using selected male and female gametophyte clones which
increase their cell number by mitosis instead of meiosis were also carried out in vitro. Juvenile sporophytes from the cross-breeding
had almost the same length and width increase rates as those of the control. The fact that vegetative gametophytes can be
purposely selected, propagated quickly, cross-bred, with the resulting sporophytes having almost the same characteristics
leads to a new way to select desiredUndaria strains for long time use without losing the desired economic characteristics.
Contribution No. 2696 from the Institute of Oceanology, Chinese Academy of Sciences.
Supported by National Natural Science Foundation of China. 相似文献
2.
Preliminary characterization of some Argentinean Artemia populations from La Pampa and Buenos Aires provinces 总被引:2,自引:0,他引:2
Rosa Graciela Cohen Francisco Amat Francisco Hontoria Juan Carlos Navarro 《International Journal of Salt Lake Research》1999,8(4):329-340
Artemia populations were sampled in four hypersaline ecosystems in continental evaporitic basins from the Argentinean provinces of
La Pampa and Buenos Aires. Biometrics of cysts and nauplii were performed. The morphometrics of adult females under standard
culture conditions, studied by multivariate discriminant analysis, provided evidence that these populations belonged to the
speciesA. persimilis; this was further supported by cross-breeding tests established between one of these populations (Salinas Grandes de Hidalgo,
province of La Pampa) and a population ofA. franciscana from San Francisco Bay (California, USA), which showed reproductive isolation and barriers to gene flow between both populations. 相似文献
3.
When cultured under certain environmental coriditions (25℃, light intensity 80 μmol/m~2 ·s, LD12/12, in enriched seawater medium with 7 × 10~(-4)mol/L NO_3,-N, 1.56× 10~(-4)mol/L, PO_4-P and supplementsof other elements like Mn, Fe, I, etc.), mate and female gametophytes of U pinnatifida keptgrowing vegetatively and propagated fast at average daily fresh weight increase rate of about 20%. Theempirical formula G_m=G_o. 3~m was established to estimate the output of vegeative gametophytes. Vigorousvegetative gametophyte cells began to form reproductive structures (oogonium and spermatangium, whenthe tmperature was lower than 25℃ and other environmental factors wrre kept optimal. The sufficientsupply of gametophyte cells provided enough seeds for raising Undaria sporelings on prodiction scale.Controlled cross-breeding experiments using selcted male and female gametophyte clones which increasetheir cell number by mitosis instead of meiosis were also carried out in vitro. Juvenile sporophytes fromthe cro 相似文献
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