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The construction of enrichment library proves to be one of the efficient approaches for isolating microsatellites in this
study. The genomic DNA of sea cucumber was digested with HaeIII and size-selected DNA fragments (250–700 bp) were ligated to an adaptor. Microsatellite-containing sequences were captured
by using a combination of GA and CA probes, which were attached to a nylon membrane. The microsatellite enrichment library
constructed in this study consisted of approximately 700 clones. Two hundred and thirty-two clones reacted positively after
the library screening procedure. Of the 50 clones sequenced, all contained at least one microsatellite and one duplicate clone
was found. Approximately 86% of the sequenced fragments permitted to design primers for sequence tagged microsatellite site
(STMS). 相似文献
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An Efficient Procedure for Isolating Microsatellite DNAs from Sea Cucumber (Apostichopus japonicus) 总被引:1,自引:0,他引:1
HU Jingjie ZHAN Aibin LU Wei HU Xiaoli and BAO Zhenmin* Laboratory of Marine Genetics Breeding 《中国海洋大学学报(英文版)》2007,6(1):12-15
1 Introduction Microsatellite DNAs, also called simple sequence re-peats (SSRs), are stretches of DNA, consisting of tan-demly repeating mono-, di-, tri-, tetra- or penta-nucleotide units. They are evenly distributed throughout the ge-nomes of most eukaryotic species (Tohn et al., 2000). Their high information content about the divergence of genome, which is directly related to the number of alleles at each locus, and the ease of PCR assays make them ex-cellent molecular markers (Powell … 相似文献
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