全文获取类型
收费全文 | 1232篇 |
免费 | 170篇 |
国内免费 | 405篇 |
专业分类
测绘学 | 130篇 |
大气科学 | 169篇 |
地球物理 | 181篇 |
地质学 | 722篇 |
海洋学 | 283篇 |
天文学 | 11篇 |
综合类 | 101篇 |
自然地理 | 210篇 |
出版年
2024年 | 4篇 |
2023年 | 19篇 |
2022年 | 49篇 |
2021年 | 48篇 |
2020年 | 59篇 |
2019年 | 83篇 |
2018年 | 53篇 |
2017年 | 64篇 |
2016年 | 56篇 |
2015年 | 72篇 |
2014年 | 108篇 |
2013年 | 119篇 |
2012年 | 87篇 |
2011年 | 82篇 |
2010年 | 81篇 |
2009年 | 76篇 |
2008年 | 69篇 |
2007年 | 69篇 |
2006年 | 78篇 |
2005年 | 62篇 |
2004年 | 72篇 |
2003年 | 47篇 |
2002年 | 56篇 |
2001年 | 43篇 |
2000年 | 40篇 |
1999年 | 28篇 |
1998年 | 26篇 |
1997年 | 37篇 |
1996年 | 22篇 |
1995年 | 14篇 |
1994年 | 15篇 |
1993年 | 8篇 |
1992年 | 14篇 |
1991年 | 10篇 |
1990年 | 6篇 |
1989年 | 5篇 |
1988年 | 7篇 |
1987年 | 3篇 |
1986年 | 4篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1977年 | 1篇 |
排序方式: 共有1807条查询结果,搜索用时 15 毫秒
1.
Kaizad F. Patel Ivan J. Fernandez Sarah J. Nelson Stephen A. Norton Cheryl J. Spencer 《水文研究》2021,35(5):e14147
The Bear Brook Watershed in Maine (BBWM) is a long-term research site established to study the response of forest ecosystem function to environmental disturbances of chronic acidic deposition and ecosystem nitrogen enrichment. Starting in 1989, the West Bear (treated) watershed received bimonthly applications of ammonium sulfate [(NH4)2SO4] fertilizer from above the canopy, whereas East Bear (reference) received ambient deposition. The treatments were stopped in 2016, marking the beginning of the recovery phase. Research at the site has focused on soils, streams, and vegetation. Here, we describe data collected over three decades at the BBWM—input and stream output nutrient fluxes, quantitative soil pits and soil chemistry, and soil temperature and moisture. 相似文献
2.
在建立斑节对虾实验室养殖模式的基础上,对野外采集的有白斑综合症病毒(WSSV)病典型症状的斑节对虾初步纯化其病毒,并进行WSSV的PCR检测,得到WSSV的感染用样品。对健康斑节对虾分别进行浸浴感染,投喂感染和注射感染。对感染死亡个体进行WSSA的PCR检测和细菌检测,证实WSSV感染性和致死性。浸浴感染、投喂感染和注射感染的感染量分别为4mL/L、0.2g/10g虾体、1/2稀释液0.05mL/10g虾体,死亡开始时间分别为16d、42h、28h,三种感染模式最终死亡率100%,从开始死亡到全部死亡延续时间分别为15d、82h、44h。 相似文献
3.
APPLICATION OF GEOGRAPHICAL PARAMETER DATABASE TO ESTABLISHMENT OF UNIT POPULATION DATABASE 总被引:1,自引:1,他引:0
Now GIS is turning into a good tool in handling geographical, economical, and population data, so we can obtain more and more information from these data. On the other hand, in some cases, for a calamity, such as hurricane, earthquake, flood, drought etc., or a decision-making, such as setting up a broadcasting transmitter, building a chemical plant etc., we have to evaluate the total population in the region influenced by a calamity or a project. In this paper, a method is put forward to evaluate the population in such special region. Through exploring the correlation of geographical parameters and the distribution of people in the same region by means of quantitative analysis and qualitative analysis, unit population database (1km× 1km) is established. In this way, estimating the number of people in a special region is capable by adding up the population in every grid involved in this region boundary. The geographical parameters are obtained from topographic database and DEM database on the scale of 相似文献
4.
5.
对虾一种无包涵体杆状病毒病原的PCR检测 总被引:4,自引:0,他引:4
为建立对虾无包涵体杆状病毒的快速诊断技术,应用PCR技术分别对暴发性流行病发生前、流行中和发病后的对虾样品进行了检测.根据已克隆的对虾杆状病毒部分基因组序列而设计的引物能特异性地扩增出靶DNA片段,最低可以检测1pg的病素DNA.由典型发病症状对虾的胃、腮、肝胰腺、中肠、游泳足、肌内和心脏等器官和组织中成功地检测到病毒.不同组织和器官经扩增得到的信号强弱不同:病虾的胃扩增得到的信号最强,中肠、肌肉、心脏和游泳足次之,腮和肝胰腺信号最弱,说明病毒在不同组织和器官中数量及感染程度不同.在对虾发病前及发病后,用二次PCR还能检测表面不发病呈隐性感染状态的对虾携带的病毒;而对野生健康虾的检测结果为阴性.研究表明,PCR是检测对虾暴发性流行病快速、灵敏而准确的方法,对病毒病的早期诊断、防治和高健康对虾品种的选育具有指导意义. 相似文献
6.
Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection. 相似文献
7.
Hayes RA Regondi S Winter MJ Butler PJ Agradi E Taylor EW Kevin Chipman J 《Marine environmental research》2004,58(2-5):665-669
Metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. We sequenced chub (Leuciscus cephalus) metallothionein cDNA which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. We then used the extended primer method to develop an accurate quantitative competitive RT-PCR assay for metallothionein mRNA. RT-PCR was used to measure metallothionein mRNA in feral chub from a range of field sites, with different levels of heavy metal pollution, in the West Midlands, UK. Measurements were complemented by analysis of liver and gill metallothionein protein by capillary electrophoresis. There was no significant difference in the metallothionein protein levels between fish of different rivers and there was no evidence of elevation of mRNA at the sites of highest metal exposure. The level of metal exposure (e.g. zinc, nickel and cadmium each ranging between 15 and 28 microg/l ) at the pH (7.5-8.5) of these rivers appears insufficient to elevate hepatic or gill metallothionein in chub. A lack of elevation of hepatic metallothionein mRNA in chub exposed to zinc, copper and manganese for 24 h and 10 days in the laboratory also suggests a non-responsiveness of this species. 相似文献
8.
用16S rRNA基因的内切酶图谱快速鉴别几种对虾病原菌 总被引:4,自引:0,他引:4
坎普氏弧菌是青岛海洋大学生物系微生物实验室于1989-1990年自对虾养殖场中国对虾红腿病心脏及血淋巴中分离并鉴定的菌株,副溶血菌和溶藻胶弧菌两菌株于1994年9月得自中国科学院微生物研究所,为建立快速、准确的中国对虾病原菌的诊断技术,根据几种细菌的16SrRNA基因的序列,设计并合成该基因的多聚酶反应的引物PL1和PL2。并用该对引物分别从坎普氏弧菌、副溶血弧菌和溶藻胶弧菌的DNA要品中扩增出分 相似文献
9.
Diagnosis of iridovirus in large yellow croaker by PCR 总被引:1,自引:0,他引:1
A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection. 相似文献
10.
基于AHP法和灰色模式识别理论的海底管道系统路由定量风险评估 总被引:3,自引:1,他引:3
根据海底管道路由潜在风险的特点及风险类型,提出了一种将层次分析法(AHP法)和灰色模式识别理论相结合的海底管道系统路由定量风险评估方法,该方法利用AHP确定风险评价指标体系,运用灰色模式识别理论,建立识别结果标准,并结合实际工程进行计算,计算结果表明该方法是可行的. 相似文献