贝类精子质量检测与评价方法的研究Ⅲ:FDA-PI双荧光复染法检测马氏珠母贝精子的存活率

将马氏珠母贝新鲜精液和精子水浴致死精液配制含有不同精子死亡率(0%、20%、40%、60%、80%)的5浓度梯度检测样品,并作为理论精子死亡率,采用二乙酰荧光素(Fluorescein diacetate,FDA)-碘化丙啶(Propidium iodide,PI)双荧光复染法检测精子存活率和质膜完整性。结果表明,FDA和PI能有效区分活精子和死精子,活精子发出绿色荧光,死精子发出红色荧光。各样品实测精子死亡率和理论死亡率之间呈极显著正相关,相关系数r=0.99(p<0.01)。FDA-PI双荧光复染法检测马氏珠母贝精子存活率,灵敏度高,重复性好,准确有效。     

Application of Tracer Experiments to Predict Leakage Channel-An Example of a Power Plant in Zhen'An

Many water conservancy and hydropower projects are located in karst areas of which complicated structure would cause significant risks to water conservancy and hydropower projects, so identifying the presence or absence of pipeline leaks is one of important basic conditions for water conservancy and hydropower project. Determining the existence of leakage channels has important applications tracer diffusion of water tracer experiment, this paper is the experimental investigation of tracer leakage passage of a hydropower station in West China, determining the existence of leakage passage can be worked through experimental investigation on part of the left abutment of the hydropower predict leakage passage to