Heat-shock protein 70 expression in shrimp Fenneropenaeus chinensis during thermal and immune-challenged stress

Using western immunoblotting, we obtained heat-shock protein 70 (HSP70) induction data and distribution in different tissues from shrimp Fenneropenaeus chinensis during thermal and immune-challenged stresses. This is probably the first report of the effects of various stressors on the expression of HSP70 in shrimp.HSP70 was prominently induced in hepatopancreas and gills, but not in muscle, eyestalk and hemolymph, when the shrimp were exposed to heat shock and Vibrio anguillavium-challenged stresses. Cold shock and WSSV treatment had no significant effects on the levels of HSP70 expression in all tissues examined. HSP70 induction was greatest after 2 h exposure to heat shock stress, which was elevated after acute heat shock exposure of 10℃ above ambient temperature.     

论虚拟企业

网络的出现和广泛运用，以及企业间的跨越了行业间的藩篱，推动了业际化的进程；业际化与网络相结合促使企业组织形式发生质变，出现了虚拟企业。与大工业时代的企业相比较，虚拟企业具有了全新的特征、类型、经济形式和生产模式，其经济效益提升到一个新的平台。     

试论企业组织结构--电子商务企业与传统企业的比较

网络时代的到来，对传统企业的组织结构、运作模式、管理方法均提出了严峻的挑战。传统的企业组织结构是适应大工业生产而形成的等级组织结构形式，它有很大的局限性；电子商务企业的组织结构是适应网络社会而形成扁平化、双向多对多、以信息为基础的新型组织结构形式。电子商务企业以其组织结构扁平化和中空化，决策分散化，运作虚拟化，灵活高效为特点，它将成为传统企业在电子商务发展阶段变革的方向和目标。     

景宁县国土资源局 早准备 早部署 有效地组织抗洪

6月18日以来，景宁县普降大暴雨，洪水；中毁道路、房屋、桥梁、电力通讯设备等，造成重大经济损失。截止23日统计，这次洪灾共引发滑坡、崩塌、泥石流等各类地质灾害106起。在这次抗洪中，县国土资源局早准备、早部署，有效地组织抗洪，全局出动抗洪23次59人。县乡村各级干部战斗在抗洪一线，特别是负责地质灾害隐患点的乡镇村干部和国土资源所工作人员高度重视，紧急撤离63个隐患点中的1503人，     

双鸭山市举行首次征地听证会

双鸭山市国土资源局严格履行法定程序，切实保护被征地农村集体经济组织和农户的知情权，日前，该市举行了首次征地听证会。     

2种亚洲龙鱼的D-Loop序列结构分析

采用PCR技术对2种亚洲龙鱼的mtDNAD_Loop全序列进行扩增和测序,序列结构分析和序列同源性比对结果表明,2种亚洲龙鱼的mtDNAD_Loop在靠近5’端有3个终止相关序列TAS(Ⅰ、Ⅱ、Ⅲ),靠近D_Loop的3’端有4个保守区域CSB1、CSB2、CSB3、CSB-D。在终止相关序列和保守区域之间是连续重复区域。经DNASP4.0软件分析,全序列中检测出多态位点数(S)为26,其中有17个转换,核苷酸多样性(Pi)为0.013,平均核苷酸差异数(K)为17.333。     

量子化学方法研究氯代苯酚类化合物对水生物毒性的构效关系

对20个氯代苯酚类化合物进行了CNDO/2量子化学计算,讨论了化合物电子结构与其对水生物翻车鱼(Bluegill)、花鳉鱼(Guppy)、和虾(Shrimp)半致死量之间的关系,分别获得了表示其构效关系的三个线性方程,它们的显著性均远高于α=0.01水平。结果表明:氯代苯酚化合物苯环上碳原子的兀电荷之和(∑Qπ_R)越大苯环上碳原子的净电荷之和(∑Q_R)越大,或LUMO轨道能(E_(LUMO))越低,均使化合物对Bluegill和Guppy水生物的毒性增大;而化合物苯环上羟基邻位的碳原子的兀电荷布居(P_3)越大,同时HOMO轨道能E_(HOMO)越高,则化合物对Shrimp毒性也越大。据此,可预测氯代苯酚类化合物对上述三种水生物的毒性。     

鲻Mugil cephalus L.与尖头酸Liza tade（Forskal）骨骼系统的比较

对南海产的鲻和尖头骼系统作了比较观察，结果表明：两种鱼脑颅都较宽且扁；其鼻骨、围眶骨、翼耳骨、上耳骨，咽颅中的前上颌骨、上颌骨、腭骨、中翼骨、后翼骨、尾舌骨、下咽骨，肩带骨中的主后颞骨、后颞骨、上颞骨、上匙骨、匙骨，以及腰带骨等特征区别明显，其中某些特征可作为属间或种间的鉴别依据。     

CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.     

郑州市矿产资源整合工作再掀高潮

3月14日,郑州市政府组织郑州市发改委、国土局、安监局、煤炭局、公安局、环保局、监察局等部门和有关县(市)、44个镇的主要领导计160余人,在巩义市召开了矿产资源整合现场会,再次掀起全市矿产资源整合高潮.