条斑紫菜酵母双杂交文库的构建及PyMAPK5互作蛋白的筛选

为进一步研究条斑紫菜促分裂原活化激酶家族PyMAPK5的下游互作蛋白,理解其生物学功能,本研究通过酵母双杂交的方法进行其相互作用蛋白的筛选。提取不同温度和失水逆境胁迫下的RNA,利用Invitrogen体系构建条斑紫菜酵母双杂交cDNA文库,其库容为1.44×107CFU,重组率为91.8%。以pGBKT7-PyMAPK5为诱饵蛋白载体,利用共转化方法,从文库中筛选得到26个与PyMAPK5互作的候选蛋白。候选蛋白集中在光系统II相关蛋白、捕光蛋白、微管蛋白、ATP酶、GTP结合蛋白及假设蛋白等。微管蛋白、捕光蛋白、光系统II蛋白一对一验证结果为阳性,表明在酵母体内存在互作。本研究为阐明条斑紫菜PyMAPK5与其互作蛋白的关系及解析PyMAPK5下游作用机制奠定了基础。     

Stream formation in W Serpentis-type binaries

By the method proposed by Nazarenko (1993) and using observational data obtained by different authors, the physical conditions in the stream in the neighbourhood of the inner Lagrangian point L₁ were calculated for three interacting binary systems of W Ser-type: β Lyr, V367 Cyg, RY Sct. It is shown, that as a result of the evolutional expansion of the mass losing component the deep layers of its atmosphere reach the neighbourhood of the first Lagrangian point L₁ and a stream of axial symmetric shape is formed. Its radius is comparable to the dimension of the mass losing component. The rate of the mass transfer increases up to 10⁻⁵ M_⊙/yr. The calculated parameters of the stream in the neighbourhood of the point L₁ explain the main observational properties of the investigated systems (existence of extended circumstellar matter with a developed structure, high mass transfer rate).     

The ROSAT Bright Survey: I. Identification of an AGN sample with hard ROSAT X-ray spectra

The ROSAT Bright Survey (RBS) aims to completely optically identify the more than 2000 brightest sources detected in the ROSAT all-sky survey at galactic latitudes |b| > 30° (excluding LMC, SMC, Virgo cluster). This paper presents a subsample of 66 bright point-like ROSAT survey sources with almost hard PSPC spectra, the hardness ratio HR1 is > 0.5 for most of the sources. Teh subsample could be nearly completely identified by low-resolution optical spectroscopy with the following breakdown into object classes: 31 Seyfert galaxies, 22 BL Lac candidates, 5 clusters of galaxies, 1 cataclysmic variable, and 5 bright stars. Only one object remained unidentified and one X-ray source was a spurious detection. The redshift distrbution peaks around 0.06 for the Seyferts and around 0.13 for the BL Lac candidates. Observations with medium spectral resolution were obtained for most of the new Seyfert galaxies. A large fraction (20 objects) are type 1 Seyfert galaxies, the other fraction includes Seyfert galaxies of type 1.5 – 1.8 (5 objects), two LINERs, and 4 possible narrow-line Seyfert 1 galaxies (NLS1). About one third of the new Seyfert's have nearby companion galaxies displaying either emission or absorption lines at the same redshift. Among them are a couple of systems showing direct morphological evidence for interaction. The large fraction of interacting galaxies among our sample suggests a scenario where interaction is the main trigger of AGN activity.     

Galaxy activity and interaction: the optical view

The relevance of interaction to the presence of Seyfert or starburst activity in the nuclei of galaxies is examined. We focus the attention on the close environments of spectroscopically selected samples of Seyfert-1, Seyfert-2 and starburst galaxies. In particular the results of a statistical procedure aimed at finding physical pairs are tested with redshift measurements of the identified pairs. Seyfert and starburts galaxies show an excess of physical companions compared to normal galaxies. This excess tends to increase as one moves from Seyfert to starburst galaxies.     

基于交互多模型的水下目标跟踪方法

According to the requirements of real-time performance and reliability in underwater maneuvering target tracking as well as clarifying motion features of the underwater target, an interacting multiple model algorithm based on fuzzy logic inference （FIMM） is proposed. Maneuvering patterns of the target are represented by model sets, including the constant velocity model （CA）, the Singer mode~, and the nearly constant speed horizontal-turn model （HT） in FIMM technology. The simulation results show that compared to conventional IMM, the reliability and real-time performance of underwater target tracking can be improved by FIMM algorithm.     

含砾石土壤降雨入渗过程模拟

在室内模拟降雨试验的基础上,利用多重相互作用连续体(MINC)组件,对含砾石土壤降雨入渗过程进行了数值模拟,以期深入理解含砾石土壤中的水土过程.研究结果表明,土壤中砾石的存在能够导致土壤水分饱和度增加,使得土壤总入渗量以及降雨入渗深度降低.含砾石土壤中存在一个陡的湿润锋面,在湿润锋面上土壤水分饱和度急剧下降.MINC组件可以很好地模拟含砾石土壤降雨入渗过程,其对含砾石土壤湿润锋和土壤含水率变化的模拟值与实测值吻合较好.降雨期间土壤水分饱和度沿土壤剖面的变化可用来解释含砾石土壤降雨入渗深度降低的原因.     

UGC 7388: A galaxy with two tidal loops

We present the results of our spectroscopic and morphological studies of the galaxy UGC 7388 with the 8.1-m Gemini North telescope. Judging by its observed characteristics, UGC 7388 is a giant late-type spiral galaxy seen nearly edge-on. The main body of the galaxy is surrounded by two faint (μ _B ～ 24 ^m /□″ and μ _B ～ 25 _· ^m 5/□″) extended (～ 20–30 kpc) loop-like structures. A large-scale rotation of the brighter loop about the main galaxy has been detected. We discuss the assumption that the tidal disruption of a relatively massive companion is observed in the case of UGC 7388. A detailed study and modeling of the observed structure of this unique galaxy can give important information about the influence of the absorption of massive companions on the galactic disks and about the structure of the dark halo around UGC 7388.     

Bimodal Structure of Gas Streams in Interacting Binaries

There are two modes of gas streams in close binary systems: geometrically thick for low mass transfer rate or geometrically thin for higher mass transfer. Geometrical thickness of the streams is not proportional to the amount of transferred mass. The limit between the two possibilities is discussed.     

Evolution and destruction of bars

We summarize here some ongoing work by our group on the formation and evolution of barred galaxies. We first discuss the evolution of bars in isolated disc galaxies. Then we address the problem of bar destruction during interactions and mergers. Finally we describe work on generation and regeneration of bars in interactions. This revised version was published online in August 2006 with corrections to the Cover Date.     

基于交互多模型的组合导航观测异常影响控制

为了控制观测值异常对组合导航精度的影响,提出基于交互多模型的观测数据异常影响控制算法,即建立观测正常和异常的多模型集,各模型间通过马尔可夫模型进行切换,当观测值正常时,正常模型起作用;观测值异常时,故障模型起作用,从而可有效地抑制观测数据异常对滤波精度的影响。