合浦珠母贝26S蛋白酶体S4,S7亚基基因片段的分离与分析

26S蛋白酶体是真核生物中一种具有ATP依赖性的蛋白酶复合体,主要通过泛肽途径选择性降解细胞内与代谢调控、细胞周期有关的功能蛋白及异常蛋白,参与多种细胞活动的调控过程。26S蛋白酶体由具有催化活性的20S亚复合体和一个具有调节作用的19S亚复合体组成,其中19S亚复合体中的ATP酶亚基是调节26S蛋白酶体活性的重要组件。本通过简并引物PCR手段,从软体动物合浦珠母贝(Pinctada fucata)中扩增到参与构成19S亚复合体的S4和S7(MSS1)两个亚基的基因片段。这两个基因片段所编码的ATP酶组件包含有Gx4GKT,DEID,SAT和H／QRxGRxxR等26S蛋白酶体ATP酶亚基的共同功能基序。这是首次在软体动物中报道26S蛋白酶体的ATP酶亚基基因序列,为研究软体动物中26S蛋白酶体的结构与功能奠定了分子基础。     

Exposure ages from relict lateral moraines overridden by the Fennoscandian ice sheet

Lateral moraines constructed along west to east sloping outlet glaciers from mountain centred, pre-last glacial maximum (LGM) ice fields of limited extent remain largely preserved in the northern Swedish landscape despite overriding by continental ice sheets, most recently during the last glacial. From field evidence, including geomorphological relationships and a detailed weathering profile including a buried soil, we have identified seven such lateral moraines that were overridden by the expansion and growth of the Fennoscandian ice sheet. Cosmogenic ¹⁰Be and ²⁶Al exposure ages of 19 boulders from the crests of these moraines, combined with the field evidence, are correlated to episodes of moraine stabilisation, Pleistocene surface weathering, and glacial overriding. The last deglaciation event dominates the exposure ages, with ¹⁰Be and ²⁶Al data derived from 15 moraine boulders indicating regional deglaciation 9600 ± 200 yr ago. This is the most robust numerical age for the final deglaciation of the Fennoscandian ice sheet. The older apparent exposure ages of the remaining boulders (14,600-26,400 yr) can be explained by cosmogenic nuclide inheritance from previous exposure of the moraine crests during the last glacial cycle. Their potential exposure history, based on local glacial chronologies, indicates that the current moraine morphologies formed at the latest during marine oxygen isotope stage 5. Although numerous deglaciation ages were obtained, this study demonstrates that numerical ages need to be treated with caution and assessed in light of the geomorphological evidence indicating moraines are not necessarily formed by the event that dominates the cosmogenic nuclide data.     

Effects of pulsed nutrient inputs on phytoplankton assemblage structure and blooms in an enclosed coastal area

The response of phytoplankton assemblage structure to terrestrial nutrient inputs was examined for the Gulf of Kalloni in the Northern Aegean Sea, a productive semi-enclosed coastal marine ecosystem. The study was focused on a typical annual cycle, and emphasis was placed on the comparative analysis between blooms developing after significant nutrient inputs from the watershed, and naturally occurring blooms. Baseline information was collected on a monthly basis from a network of stations located in the oligotrophic open sea and the interior and more productive part of the embayment. Intensive sampling was also carried out along a gradient in the vicinity of a river which was the most important source of freshwater and nutrient input for the Gulf. Phytoplankton assemblage structure was analyzed from 188 samples using diversity indices (Shannon and Average Taxonomic Distinctness), multivariate plotting methods (NMDS), multivariate statistics (PERMANOVA), and canonical correspondence analysis (CCA). Three characteristic assemblages were recognized: (1) an autumn assemblage developed under nutrient depleted conditions, having low diversity due to the dominance of two small diatoms, (2) a winter bloom of the potentially toxic species Pseudo-nitzschia calliantha occurring immediately after a nutrient peak and characterized by very low diversity, and (3) a naturally occurring early summer bloom of centric diatoms with relatively high diversity. The results of the study support the view that moderate nutrient inputs may have a beneficial effect on the functioning of coastal ecosystems, stimulating the taxonomic diversity through the growth of different taxonomic groups and taxa. On the other hand, a sudden pulse of high nutrient concentrations may greatly affect the natural succession of organisms, have a negative effect on the diversity through the dominance of a single species, and can increase the possibility of a harmful algal bloom development.     

Periglacial climate at the 2.5 Ma onset of Northern Hemisphere glaciation inferred from the Whippoorwill Formation, northern Missouri, USA

The Whippoorwill Formation is a gleyed diamicton that is present locally within bedrock depressions beneath the oldest glacial till in northern Missouri, USA. Stratigraphy, paleomagnetism, and cosmogenic-nuclide burial ages show that it was deposited between the Matuyama-Gauss magnetostratigraphic boundary at 2.58 Ma and the first advance of the Laurentide ice sheet into Missouri at 2.47 ± 0.19 Ma. High cosmogenic-nuclide concentrations also show that the constituents of the Whippoorwill Formation experienced long exposure at a stable landscape surface with erosion rates of 1-2 m/Ma. However, cosmogenic-nuclide concentrations are invariant with depth below the Whippoorwill Formation surface, indicating active mixing of the soil profile shortly before burial by till. The Whippoorwill Formation retains numerous features indicative of cryoturbation. Therefore, we interpret it as a buried Gelisol, a soil formed under periglacial conditions in the presence of permafrost. At the onset of Northern Hemisphere glaciation, climate cooling established permafrost conditions and accelerated erosion by inducing landscape instability. Thus, weathered regolith materials were mobilized and redeposited by gelifluction shortly before the ice sheet overrode the landscape.     

Erosions on the southern Tibetan Plateau: Evidence from in-situ cosmogenic nuclides 10Be and 26Al in fluvial sediments

Investigating topographic and climatic controls on erosion at variable spatial and temporal scales is essential to our understanding of the topographic evolution of the orogen.In this work,we quantified millennial-scale erosion rates deduced from cosmogenic 10Be and 26Al concentrations in 15 fluvial sediments from the mainstream and major tributaries of the Yarlung Zangbo River draining the southern Tibetan Plateau (TP).The measured ratios of 26Al/10Be range from 6.33 ± 0.29 to 8.96 ± 0.37,suggesting steady-state erosion processes.The resulted erosion rates vary from 20.60 ± 1.79 to 154.00 ± 13.60 m Myr-1,being spatially low in the upstream areas of the Gyaca knickpoint and high in the downstream areas.By examining the relationships between the erosion rate and topographic or climatic indices,we found that both topography and climate play significant roles in the erosion process for basins in the upstream areas of the Gyaca knickpoint.However,topography dominantly controls the erosion processes in the downstream areas of the Gyaca knickpoint,whereas variations in precipitation have only a second-order control.The marginal Himalayas and the Yarlung Zangbo River Basin (YZRB) yielded significantly higher erosion rates than the central plateau,which indicated that the landscape of the central plateau surface is remarkably stable and is being intensively consumed at its boundaries through river headward erosion.In addition,our 10Be erosion rates are comparable to present-day hydrologic erosion rates in most cases,suggesting either weak human activities or long-term steady-state erosion in this area.     

Polarization-mode separation and the emission geometry of pulsar 0823 + 26: A new pattern of pulsar emission?

We explore the detailed polarization behaviour of pulsar 0823 + 26 using the technique of constructing partial ‘mode-separated’ profiles corresponding to the primary and secondary polarization modes. The characteristics of the two polarization modes in this pulsar are particularly interesting, both because they are anything but orthogonal and because the secondary mode exhibits a structure seen neither in the primary mode nor in the total profile. The new leading and trailing features in the secondary mode, which appear to represent a conal component pair, are interpreted geometrically on the basis of their width and the associated polarization-angle traverse as an outer cone. If the secondary-mode features are, indeed, an outer cone, then questions about the significance of the pulsar’s postcursor component become more pressing. It seems that 0823 + 26 has a very nearly equatorial geometry, in that both magnetic poles and the sightline all fall close to the rotational equator of the star. We thus associate the postcursor component with emission along those bundles of field lines which are also equatorial and which continue to have a tangent in the direction of our sight line for a significant portion of the star’s rotation cycle. It seems that in all pulsars with postcursor components, this emission follows the core component, and all may thus have equatorial emission geometries. No pulsars with precursors in this sense — including the Crab pulsar — are known. The distribution of power between the primary and secondary modes is very similar at both 430 and 1400 MHz. Our analysis shows that in this pulsar considerable depolarization must be occurring on time scales that are short compared to the time resolution of our observations, which is here some 0.5–1.0 milliseconds. One of the most interesting features of the modeseparated partial profiles is a phase offset between the primary and secondary modes. The secondary-mode ‘main pulse’ arrives some 1.5 ± 0.1‡ before the primary-mode one at 430 MHz and some 1.3 +0.1 ‡ at 21 cm. Given that the polar cap has an angular diameter of 3.36‡, we consider whether this is a geometric effect or an effect of differential propagation of the two modes in the inner magnetosphere of the pulsar.     

26Al/10Be等时线埋藏测年法基本原理与应用简介

介绍了26Al/10Be等时线埋藏测年法的基本原理及其主要应用范围。将应被同时埋藏的一组石英矿物样的26Al-10Be浓度拟合成等时线,从其斜率解得样品的埋藏年代。在如下情况下该法可克服未知参数多于制约方程的困难:1)冰碛物-古土壤序列中古土壤形成年代的研究,以规避继承核素(inherited nu-clide)的干扰;2)因样品埋藏不够深引起的后期生成核素的干扰。此外,依据等时线拟合结果可判断样品是否符合简单恒态侵蚀模式。等时线法的引入,拓宽了埋藏测年的应用范围,并为埋藏测年数据可信度提供了一种有效的验证手段。     

Short-Term Morphological and Shoreline Changes at Trinkat Island,Andaman and Nicobar,India, After the 2004 Tsunami

The tsunami waves generated during the Sumatra-Andaman earthquake of 26 December 2004 devastated the coastal area along Trinkat Island, causing sudden changes to the morphology of the landforms. This study uses a series of satellite images to record the short-term morphological response and shoreline changes as well as the recovery of coastal land after its destruction. Results indicate that the island experienced substantial erosion and a significant reduction in land area. Shoreline erosion is more prevalent than accretion at an average linear regression rate of ～?9 m per year between 2004 and 2013. The major morphological changes at Trinkat Island were observed in coastal inlets, beaches, and bay head-lands. Straight beaches had almost recovered eight years after the tsunami; however, erosion is continually observed in other areas. Our study will help understanding the response and recovery of shorelines in Indian Ocean regions after the 2004 tsunami.     

Experimental evidence for flexibility of a building foundation supported by concrete friction piles

This article explores the possibility to measure deformations of building foundations from measurements of ambient noise and strong motion recordings. The case under study is a seven-storey hotel building in Van Nuys, California. It has been instrumented by strong motion accelerographs, and has recorded several earthquakes, including the 1971 San Fernando (M_L=6.6, R=22 km), 1987 Whittier–Narrows (M_L=5.9, R=41 km), 1992 Landers (M_L=7.5, R=186 km), 1992 Big Bear (M_L=6.5, R=149 km), and 1994 Northridge (M_L=6.4, R=1.5 km) earthquake and its aftershocks (20 March: M_L=5.2, R=1.2 km; 6 December, 1994: M_L=4.3, R=11 km). It suffered minor structural damage in 1971 earthquake and extensive damage in 1994. Two detailed ambient vibration tests were performed following the Northridge earthquake, one before and the other one after the 20 March aftershock. These included measurements at a grid of points on the ground floor and in the parking lot surrounding the building, presented and analyzed in this article. The analysis shows that the foundation system, consisting of grade beams on friction piles, does not act as a “rigid body” but deforms during the passage of microtremor and therefore earthquake waves. For this geometrically and by design essentially symmetric building, the center of stiffness of the foundation system appears to have large eccentricity (this is seen both from the microtremor measurements and from the earthquake recordings). This eccentricity may have contributed to strong coupling of transverse and torsional responses, and to larger than expected torsional response, contributing to damage during the 1994 Northridge, earthquake.