线纹海马肠道菌群结构与功能对迟钝爱德华氏菌(Edwardsiella tarda)感染的响应特征研究

细菌性肠炎对海马养殖业影响巨大, 但病原对海马肠道菌群的具体影响尚不清楚。文章利用已分离的病原细菌 Edwardsiella tarda YT1和海马细菌性肠炎模型, 结合16S rDNA高通量测序技术, 探究病原细菌侵染对海马肠道菌群的影响。结果发现, E. tarda侵染改变了海马肠道菌群的结构组成、多样性和丰度, 并显著降低了其多样性(p<0.05); 显著增加了海马肠道变形菌门(Proteobacteria)的相对丰度(p<0.05), 减少了放线菌门(Actinobacteria)、厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)的相对丰度(p<0.05); 导致致病菌爱德华氏菌属(Edwardsiella)在属水平的相对丰度极显著增加(p<0.01), 而肠道固有菌群嗜冷杆菌属(Psychrobacter)和罗氏菌属(Rothia)极显著减少(p<0.01), 以及球菌属(Macrococcus)与动球菌属(Planococcus)显著减少(p<0.05)。研究结果表明, E. tarda能通过改变海马肠道固有优势菌群的相对丰度导致菌群失调。菌群功能变化及其相关性分析表明, E. tarda可能通过显著提高细菌趋化性、鞭毛组装、ABC转运蛋白、磷酸转运酶系统以及脂多糖生物合成途径的活性(p<0.05), 抑制肠道核心菌群如嗜冷杆菌属、动球菌属和谷氨酸杆菌属的丰度及其核糖体、RNA降解、核苷酸剪切修复与脂肪酸生物合成途径的活性(p<0.05), 导致肠道菌群功能失调, 并诱发肠炎。     

线纹海马Hippocampus erectus的tlr21基因结构及其对CpG-ODN的应答特征

作为先天性免疫反应中重要的模式识别受体之一, TLR(toll-like receptor)基因家族介导的先天性免疫是脊椎动物抵御病原微生物的重要防线。研究分析了线纹海马Hippocampus erectus tlr21基因的序列特征, 并基于抗原注射实验探讨了海马tlr21的免疫功能。线纹海马tlr21基因的开放阅读框长度为2946bp, 编码981个氨基酸, 预测其编码蛋白相对分子量为246.98kDa, 理论等电点为4.78。tlr21编码蛋白主要包含1个信号肽和3个功能结构域: 胞外区具有14个富含亮氨酸重复序列的结构域(LRR), 跨膜区具有富含半胱氨酸的结构域, 胞内区则具有TIR结构域。同源性比较和系统进化分析表明, 线纹海马tlr21基因与虎尾海马Hippocampus comes tlr21基因的同源性最高, 其次与斜带石斑鱼Epinephelus coioides、牙鲆Paralichthys olivaceus、大菱鲆Scophthalmus maximus和红鳍东方鲀Takifugu rubripes的tlr21基因聚为一枝。tlr21基因在线纹海马的脑、鳃、肝、肠、肾、性腺、肌肉和育儿袋各组织均有分布, 肾脏中表达量最高。抗原注射实验结果发现, 线纹海马Tlr21对不同类型CpG寡脱氧核苷酸(CpG-ODNs)的识别能力存在差异, 其中, CpG-2007和CpG-HC4040对海马肾脏tlr21的mRNA表达量具有显著的促进作用(P<0.05)。研究表明, 线纹海马Tlr21能够通过识别含CpG序列的DNA发挥免疫识别作用, 研究结果将有助于系统认识海马免疫系统中TLR家族基因的功能特征, 为建立海马病害防治策略提供理论依据。     

海马卵甲藻病的防治研究

调查了养殖海马（Hippocampus）卵甲藻病的发病流行规律，并研究其防治方法。结果表明养殖海马在自然水温23℃以上时易发生淀粉卵甲藻病，致病死亡主要原因为淀粉卵甲藻抱囊附着在海马的鳃部引起海马呼吸困难。从而导致海马缺氧。采用含Cu^2＋药物可以一般预防和治疗海马淀粉卵甲藻病。流水及网箱养殖海马不易发生此病，也是较好的防治方法。     

Preliminary observations on breeding and rearing the seahorse Hippocampus abdominalis (Teleostei: Syngnathidae) in captivity

A small‐scale preliminary investigation into the breeding and rearing of the seahorse Hippocampus abdominalis Leeson, 1827 in New Zealand was conducted using 12 wildbroodstock. These broodstock were maintained in a hatchery for breeding and the breeding behaviours exhibited by these were observed and described. Over a period of 6 months a total of 12 broods of juveniles were produced. These were then raised on a diet consisting mainly of enriched Artemia for a period of 1 year. After this period juveniles had reached a mean ± 1 SE total length of 110.73 ±0.13 mm, mean ± 1 SE wet weight of 3.07 + 0.23 g, and were reproductively mature. The mean + 1 SE survival per brood after 1 year was 10.57 ± 3.49%.     

Natural diet of the seahorse Hippocampus abdominalis

This investigation examined the diet of adult wild seahorses, Hippocampus abdominalis Leeson 1827, from Wellington Harbour, New Zealand. Diet of seahorses (n = 59) collected from shallow subtidal macroalgal stands consisted largely of crustaceans, in particular amphipods (e.g., caprellid and ischyrocerid amphipods), caridean shrimp (i.e., Hippolyte bifidirostris), and peracarids (i.e., the mysid Tenagomysis similis). There were no differences in diet between male and female seahorses. Smaller seahorses consumed a greater amount of crustaceans than larger seahorses, as a result of the greater proportion of amphipods in their gut contents. There were some seasonal differences in diet, with amphipod consumption peaking in spring and summer, and decapod consumption lowest in autumn.     

海马人工养殖的研究

介绍海马的生态习性和试养经验,强调对仔稚鱼期投喂适口性好、营养全面的鲜活浮游甲壳类动物可促进其生长、发育,增强抗病能力,提高成活率。提出与沿海对虾育苗场、养殖场相结合,建立海马苗种供应基地和养殖技术咨询服务中心,推动海马人工养殖业发展。